Decrease in expression of maternal effect gene Mater is associated with maternal ageing in mice

CORC > 昆明动物研究所 > 昆明动物研究所 > 遗传资源与进化国家重点实验室 > 哺乳动物胚胎发育

	Decrease in expression of maternal effect gene Mater is associated with maternal ageing in mice
	Lu YQ 1,2; He XC 1,2; Zheng P[*]1,2
刊名	MOLECULAR HUMAN REPRODUCTION
	2016
卷号	22 期号:4 页码:252-260
关键词	Mater aneuploidy cohesion maternal ageing oocyte
通讯作者	zhengp@mail.kiz.ac.cn
合作状况	其它
英文摘要	STUDY HYPOTHESIS: What factors in mouse oocytes are involved in the ageing-related decline in oocyte quality? STUDY FINDING: The maternal effect gene Mater is involved in ageing-related oocyte quality decline in mice. WHAT IS KNOWN ALREADY: Premature loss of centromere cohesion is a hallmark of ageing-related oocyte quality decline; the maternal effect gene Mater (maternal antigen that embryos require, also known as Nlrp5) is required for preimplantation embryo development beyond the 2-cell stage, and mRNA expression of Mater decreases with maternal ageing. STUDY DESIGN, SAMPLES/MATERIALS, METHODS: Mater protein expression level in mature oocytes from 7 young (5-8 weeks old) to 7 old mice (41-68 weeks old) was compared by immunoblotting analysis. Wild-type and Mater-null mice were used to examine whether Mater is necessary for maintaining normal centromere cohesion by means of cytogenetic karyotyping, time-lapse confocal microscopy and immunofluorescence staining. MAIN RESULTS AND THE ROLE OF CHANCE: Mater protein is decreased in mature oocytes from old versus young mice (P = 0.0022). Depletion of Mater from oocytes leads to a reduction in centromere cohesion, manifested by precocious sister chromatid separation, enlargement of sister centromere distance and misalignment of chromosomes in the metaphase plate during meiosis I and II. LIMITATIONS, REASONS FOR CAUTION: This study was conducted in mice. Whether or not the results are applicable to human remains further elucidation. In addition, we were unable to confirm if the strain of mice (C57BL/6XSv129) at the age of 41-68 weeks old has the 'cohesin-loss' phenotype. WIDER IMPLICATIONS OF THE FINDINGS: Investigating Mater's functional mechanisms could provide fresh insights into understanding how the ageing-related oocyte quality decline occurs.
收录类别	SCI
资助信息	This work was supported by the research grant from Chinese NSFC to P.Z. (31071274).
语种	英语
内容类型	期刊论文
源URL	[http://159.226.149.26:8080/handle/152453/9530]
专题	昆明动物研究所_哺乳动物胚胎发育昆明动物研究所_遗传资源与进化国家重点实验室
作者单位	1.State Key Laboratory of Genetic Resources and Evolution, Kunming Institute of Zoology, Chinese Academy of Sciences, Kunming, Yunnan 650223, Chin 2.Yunnan Key Laboratory of Animal Reproduction, Kunming Institute of Zoology, Chinese Academy of Sciences, Kunming, Yunnan 650223, China
推荐引用方式 GB/T 7714	Lu YQ,He XC,Zheng P[*]. Decrease in expression of maternal effect gene Mater is associated with maternal ageing in mice[J]. MOLECULAR HUMAN REPRODUCTION,2016,22(4):252-260.
APA	Lu YQ,He XC,&Zheng P[*].(2016).Decrease in expression of maternal effect gene Mater is associated with maternal ageing in mice.MOLECULAR HUMAN REPRODUCTION,22(4),252-260.
MLA	Lu YQ,et al."Decrease in expression of maternal effect gene Mater is associated with maternal ageing in mice".MOLECULAR HUMAN REPRODUCTION 22.4(2016):252-260.