Modification of BECN1 by ISG15 plays a crucial role in autophagy regulation by type I IFN/interferon

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	Modification of BECN1 by ISG15 plays a crucial role in autophagy regulation by type I IFN/interferon
	XU DAICHAO 1; ZHANG TAO 1; XIAO JUAN 1; ZHU KEZHOU 1; WEI RAN 1; WU ZHEMING 1; MENG HUYAN 1; LI YING 1; Yuan JY(袁钧瑛)1
刊名	Autophagy
	2015
卷号	11 期号:4 页码:617-628
其他题名	Modification of BECN1 by ISG15 plays a crucial role in autophagy regulation by type I IFN/interferon
英文摘要	ISG15 (ISG15 ubiquitin-like modifier), a ubiquitin-like protein, is one of the major type I IFN (interferon) effector systems. ISG15 can be conjugated to target proteins (ISGylation) via the stepwise action of E1, E2, and E3 enzymes. Conjugated ISG15 can be removed (deISGylated) from target proteins by USP18 (ubiquitin-specific peptidase 18). Here we investigated the role of deISGylation by USP18 in regulating autophagy and EGFR degradation in cells treated with type I IFNs. We show that type I IFN induced expression of ISG15 leads to ISGylation of BECN1 at Lys117, as well as Lys263, Lys265, and Lys266 which competes with Lys63 ubiquitination of BECN1. We demonstrate that ISGylation of BECN1 at Lys117, as well as Lys263, Lys265, and Lys266 serve an important role in negative regulation of intracellular processes including autophagy and EGFR degradation that are critically dependent upon the activity of class III PtdIns 3-kinase. Our studies provide fundamental new mechanistic insights into the innate immunity response implemented by type I IFNs.
学科主题	生命有机化学
收录类别	SCI
原文出处	http://dx.doi.org/10.1080/15548627.2015.1023982
语种	英语
WOS记录号	WOS:000354185500005
内容类型	期刊论文
源URL	[http://ir.sioc.ac.cn/handle/331003/39502]
专题	上海有机化学研究所_生命有机化学国家重点实验室
作者单位	1.中科院上海有机化学研究所, 生物与化学交叉研究中心 2.美国哈佛大学
推荐引用方式 GB/T 7714	XU DAICHAO,ZHANG TAO,XIAO JUAN,et al. Modification of BECN1 by ISG15 plays a crucial role in autophagy regulation by type I IFN/interferon[J]. Autophagy,2015,11(4):617-628.
APA	XU DAICHAO.,ZHANG TAO.,XIAO JUAN.,ZHU KEZHOU.,WEI RAN.,...&袁钧瑛.(2015).Modification of BECN1 by ISG15 plays a crucial role in autophagy regulation by type I IFN/interferon.Autophagy,11(4),617-628.
MLA	XU DAICHAO,et al."Modification of BECN1 by ISG15 plays a crucial role in autophagy regulation by type I IFN/interferon".Autophagy 11.4(2015):617-628.