Direct infusion electrospray ionization-ion mobility-mass spectrometry for comparative profiling of fatty acids based on stable isotope labeling

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	Direct infusion electrospray ionization-ion mobility-mass spectrometry for comparative profiling of fatty acids based on stable isotope labeling
	Leng JP(冷嘉鹏)1; Guan Q(官青)1; Sun TQ(孙团起)1; Wang HY(王昊阳)1; CUI JIANLAN 1; LIU QINGHAO 1; ZHANG ZHIXU 1; Zhang ZX(张之旭)1; Zhang MY(张曼玉)1; Guo YL(郭寅龙)1
刊名	Anal. Chim. Acta
	2015
卷号	887 页码:148-154
其他题名	基于稳定同位素标记技术的电喷雾离子淌度质谱法的脂肪酸比较分析的开发
通讯作者	冷嘉鹏 ; 孙团起 ; 郭寅龙
英文摘要	A rapid method for fatty acids (FAs) comparative profiling based on carboxyl-specific stable isotope labeling (SIL) and direct infusion electrospray ionization-ion mobility-mass spectrometry (ESI-IM-MS) is established. The design of the method takes advantage of the three-dimensional characteristics of IM -MS including drift time, m/z and ion intensity, for comparison of d0-/d6-2,4-dimethoxy-6-piperazin-1-yl pyrimidine (DMPP)-labeled FAs. In particular, without chromatographic separation, the method allowed direct FAs profiling in complex samples due to the advantageous priority of DMPP in signal enhancement as well as the extra resolution that IM-MS offered. Additionally, the d0-/d6-DMPP-labeled FAs showed expected features, including very similar drift times, 6 Da mass deviations, specific reporter ions, similar MS responses, and adherence to the drift time rule regarding the influence of carbon chain length and unsaturation on relative drift times. Therefore, the introduction of isotope analogs minimized the matrix effect and variations in quantification and ensured accurate identification of non-targeted FAs by those typical features. Peak intensity ratios between d0-/d6-DMPP-labeled ions were subsequently used in relative quantification for the detected FAs. The established strategy has been applied successfully in the rapid profiling of trace free FAs between normal and cancerous human thyroid tissues. Sixteen free FAs were found with the increased level with a statistically significant difference (p < 0.05) compared to the normal tissue samples. The integrated SIL technique and ESI-IM-MS are expected to serve as an alternative tool for high-throughput analysis of FAs in complex samples. (C) 2015 Elsevier B.V. All rights reserved.
学科主题	分析化学
收录类别	SCI
原文出处	http://dx.doi.org/10.1016/j.aca.2015.06.029
语种	英语
WOS记录号	WOS:000360644100016
内容类型	期刊论文
源URL	[http://ir.sioc.ac.cn/handle/331003/39693]
专题	上海有机化学研究所_分析化学研究室
作者单位	1.中北大学 2.复旦大学上海癌症中心 3.复旦大学上海医科大学 4.中科院上海有机化学研究所, 上海质谱中心 5.安捷伦科技(中国)有限公司
推荐引用方式 GB/T 7714	Leng JP,Guan Q,Sun TQ,et al. Direct infusion electrospray ionization-ion mobility-mass spectrometry for comparative profiling of fatty acids based on stable isotope labeling[J]. Anal. Chim. Acta,2015,887:148-154.
APA	冷嘉鹏.,官青.,孙团起.,王昊阳.,CUI JIANLAN.,...&郭寅龙.(2015).Direct infusion electrospray ionization-ion mobility-mass spectrometry for comparative profiling of fatty acids based on stable isotope labeling.Anal. Chim. Acta,887,148-154.
MLA	冷嘉鹏,et al."Direct infusion electrospray ionization-ion mobility-mass spectrometry for comparative profiling of fatty acids based on stable isotope labeling".Anal. Chim. Acta 887(2015):148-154.