Effects of cooperation between translating ribosome and RNA polymerase on termination efficiency of the Rho-independent terminator

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	Effects of cooperation between translating ribosome and RNA polymerase on termination efficiency of the Rho-independent terminator
	Li, Rui 1; Zhang, Qing 1; Li, Junbai 2,3; Shi, Hualin 1
刊名	NUCLEIC ACIDS RESEARCH
	2016-04-07
卷号	44 期号:6 页码:2554-2563
英文摘要	An experimental system was designed to measure in vivo termination efficiency (TE) of the Rho-independent terminator and position-function relations were quantified for the terminator tR2 in Escherichia coli. The terminator function was almost completely repressed when tR2 was located several base pairs downstream from the gene, and TE gradually increased to maximum values with the increasing distance between the gene and terminator. This TE-distance relation reflected a stochastic coupling of the ribosome and RNA polymerase (RNAP). Terminators located in the first 100 bp of the coding region can function efficiently. However, functional repression was observed when the terminator was located in the latter part of the coding region, and the degree of repression was determined by transcriptional and translational dynamics. These results may help to elucidate mechanisms of Rho-independent termination and reveal genomic locations of terminators and functions of the sequence that precedes terminators. These observations may have important applications in synthetic biology.
收录类别	SCI
语种	英语
内容类型	期刊论文
源URL	[http://ir.iccas.ac.cn/handle/121111/36108]
专题	化学研究所_胶体、界面与化学热力学实验室
作者单位	1.Chinese Acad Sci, Inst Theoret Phys, State Key Lab Theoret Phys, Beijing 100190, Peoples R China 2.Chinese Acad Sci, Inst Chem, Beijing 100190, Peoples R China 3.Natl Ctr Nanosci & Technol, Beijing 100190, Peoples R China
推荐引用方式 GB/T 7714	Li, Rui,Zhang, Qing,Li, Junbai,et al. Effects of cooperation between translating ribosome and RNA polymerase on termination efficiency of the Rho-independent terminator[J]. NUCLEIC ACIDS RESEARCH,2016,44(6):2554-2563.
APA	Li, Rui,Zhang, Qing,Li, Junbai,&Shi, Hualin.(2016).Effects of cooperation between translating ribosome and RNA polymerase on termination efficiency of the Rho-independent terminator.NUCLEIC ACIDS RESEARCH,44(6),2554-2563.
MLA	Li, Rui,et al."Effects of cooperation between translating ribosome and RNA polymerase on termination efficiency of the Rho-independent terminator".NUCLEIC ACIDS RESEARCH 44.6(2016):2554-2563.