A Cyanine Dye to Probe Mitophagy: Simultaneous Detection of Mitochondria and Autolysosomes in Live Cells

	A Cyanine Dye to Probe Mitophagy: Simultaneous Detection of Mitochondria and Autolysosomes in Live Cells
	Liu, Ying 1,2; Zhou, Jin 1,6; Wang, Linlin 1,2; Hu, Xiaoxiao 3,4; Liu, Xiangjun 1; Liu, Meirong 1; Cao, Zehui 1; Shangguan, Dihua 1,2; Tan, Weihong 3,4,5
刊名	JOURNAL OF THE AMERICAN CHEMICAL SOCIETY
	2016-09-28
卷号	138 期号:38 页码:12368-12374
英文摘要	Mitophagy is a process in which cells remove dysfunctional mitochondria and recycle their constituents in a lysosome-dependent manner. To probe this process, two different fluorescent dyes specific for mitochondria and lysosomes, respectively, are often used in combination. However, current fluorescent dyes for lysosomes cannot distinguish mitochondria-containing autolysosomes from other lysosomes. Therefore, we herein report a cyanine dye, HQO, which can simultaneously probe mitochondria and autolysosomes in live cells by exhibiting different fluorescence properties. HQO selectively accumulates in mitochondria but then transforms to the protonated HQOH(+) form with the decrease of pH when dysfunctional mitochondria evolve into autolysosomes. Since HQO and HQOH(+) exhibit different absorption and emission with Ex/Em at 530/650 and 710/750 nm, respectively, in a low polarity environment, such as that found in micelles, they are uniquely suited to monitor mitophagy with the ability to distinguish autolysosomes from other lysosomes.
收录类别	SCI
语种	英语
内容类型	期刊论文
源URL	[http://ir.iccas.ac.cn/handle/121111/35653]
专题	化学研究所_活体分析化学实验室
作者单位	1.Chinese Acad Sci, Inst Chem, Key Lab Analyt Chem Living Biosyst, Beijing Natl Lab Mol Sci, Beijing 100190, Peoples R China 2.Univ Chinese Acad Sci, Beijing 100049, Peoples R China 3.Hunan Univ, Coll Biol, State Key Lab Chemo Biosensing & Chemometr, Mol Sci & Biomed Lab, Changsha 410082, Hunan, Peoples R China 4.Hunan Univ, Coll Chem & Chem Engn, Changsha 410082, Hunan, Peoples R China 5.Univ Florida, Dept Chem, Ctr Res Bio Nano Interface, Hlth Canc Ctr,UF Genet Inst,McKnight Brain Inst, Gainesville, FL 32611 USA 6.Natl Ctr Nanosci & Technol, CAS Ctr Excellence Nanosci, Beijing 100190, Peoples R China
推荐引用方式 GB/T 7714	Liu, Ying,Zhou, Jin,Wang, Linlin,et al. A Cyanine Dye to Probe Mitophagy: Simultaneous Detection of Mitochondria and Autolysosomes in Live Cells[J]. JOURNAL OF THE AMERICAN CHEMICAL SOCIETY,2016,138(38):12368-12374.
APA	Liu, Ying.,Zhou, Jin.,Wang, Linlin.,Hu, Xiaoxiao.,Liu, Xiangjun.,...&Tan, Weihong.(2016).A Cyanine Dye to Probe Mitophagy: Simultaneous Detection of Mitochondria and Autolysosomes in Live Cells.JOURNAL OF THE AMERICAN CHEMICAL SOCIETY,138(38),12368-12374.
MLA	Liu, Ying,et al."A Cyanine Dye to Probe Mitophagy: Simultaneous Detection of Mitochondria and Autolysosomes in Live Cells".JOURNAL OF THE AMERICAN CHEMICAL SOCIETY 138.38(2016):12368-12374.