Chemical Strategy to Stepwise Amplification of Signals in Surface Plasmon Resonance Imaging Detection of Saccharides and Glycoconjugates

	Chemical Strategy to Stepwise Amplification of Signals in Surface Plasmon Resonance Imaging Detection of Saccharides and Glycoconjugates
	Liu, Chanjuan 1,2; Wang, Xiao 1,2; Xu, Jiying 1; Chen, Yi 1,2,3
刊名	ANALYTICAL CHEMISTRY
	2016-10-18
卷号	88 期号:20 页码:10011-10018
英文摘要	A stepwise strategy Was proposed to controllably amplify surface plasmon resonance imaging (SPRi) signals and used to establish a method for sensitive detection of small saccharides and large glycoconjugates. The key is to enlarge the target analytes step by step through a cyclic recognition reaction of concanavalin A (ConA) with dextran, which can easily be integrated into SPRi, detection. The reaction is theoretically expected to proceed with infinite cycles by addition of ConA and dextran one after another, and in-practice, it allows for performing signal amplification. for up to 20 steps, which nearly reaches the limit of the propagation depth of surface plasmon waves. Because of the nonspecific adsorption effect, the maximum signals of small sugars were measured at steps 5-7 by the use of small molecules like ethanol amine to block the nonspecific adsorption. The real applicability of the method was validated by SPRi determination of either immobilized saccharides and glycoproteins or captured catcinoembryonic antigen (CEA) from patient samples with a litnit of detection down to 2.5 mu M glucose or 50 pg/mL of CEA. Awideiextenclibility of the method was confirmed by detecting other-;ConA-recognizable analytes like human IgG, Oyalbumin, a-fetoprotein, and analytes convertible to ConA- or dextranrecognizable forms. Notably, other,cyclic reactions specific to saccharides or other analytes are in theory exploitable, which will further widen the applicability of the method and strategy.
收录类别	SCI
语种	英语
内容类型	期刊论文
源URL	[http://ir.iccas.ac.cn/handle/121111/35470]
专题	化学研究所_活体分析化学实验室
作者单位	1.Chinese Acad Sci, Inst Chem, Key Lab Analyt Chem Living Biosyst, Beijing 100190, Peoples R China 2.Univ Chinese Acad Sci, Beijing 100049, Peoples R China 3.Beijing Natl Lab Mol Sci, Beijing 100190, Peoples R China
推荐引用方式 GB/T 7714	Liu, Chanjuan,Wang, Xiao,Xu, Jiying,et al. Chemical Strategy to Stepwise Amplification of Signals in Surface Plasmon Resonance Imaging Detection of Saccharides and Glycoconjugates[J]. ANALYTICAL CHEMISTRY,2016,88(20):10011-10018.
APA	Liu, Chanjuan,Wang, Xiao,Xu, Jiying,&Chen, Yi.(2016).Chemical Strategy to Stepwise Amplification of Signals in Surface Plasmon Resonance Imaging Detection of Saccharides and Glycoconjugates.ANALYTICAL CHEMISTRY,88(20),10011-10018.
MLA	Liu, Chanjuan,et al."Chemical Strategy to Stepwise Amplification of Signals in Surface Plasmon Resonance Imaging Detection of Saccharides and Glycoconjugates".ANALYTICAL CHEMISTRY 88.20(2016):10011-10018.