Synthetic Short Peptides for Rapid Fabrication of Monolayer Cell Sheets

	Synthetic Short Peptides for Rapid Fabrication of Monolayer Cell Sheets
	Ma, Yinan 1,2; Li, Zhibo 1,3; Numata, Keiji 2
刊名	ACS BIOMATERIALS-SCIENCE & ENGINEERING
	2016-04-01
卷号	2 期号:4 页码:697-706
关键词	oligopeptides chemoenzymatic synthesis enzymatic activity monolayer cell sheet
英文摘要	Cell sheets are useful materials in regenerative medicine; however, the cell sheet fabrication processes developed to date are associated with several crucial challenges. The aim of this study was to develop a new and simple method for the rapid and efficient fabrication of transferable monolayer cell sheets. Chemoenzymatic synthesis mediated by proteinase K was used to synthesize short cooligopeptides.for cell sheet fabrication, which showed high yield, well-defined structures, and a controllable composition. These co-oligopeptides predominantly adopted a random coil conformation in buffer. Histidine/cysteine co-oligopeptides with a disordered secondary structure displayed cysteine content-dependent esterase activity and cysteine content-independent protease activity. Taking advantage of this enzymatic activity, confluent cell monolayers were detached by simply adding the cooligopeptides solution to the culture media, and then an intact monolayer cell sheet was prepared with high cell viability and reattachment ability. The method proposed herein for preparing monolayer cell sheets represents a novel concept by using oligopeptides with enzymatic activity that show applied potential in cell sheet technology for tissue engineering and regenerative medicine.
收录类别	SCI
语种	英语
内容类型	期刊论文
源URL	[http://ir.iccas.ac.cn/handle/121111/36007]
专题	化学研究所_高分子物理与化学实验室
作者单位	1.Chinese Acad Sci, Inst Chem, Lab Polymer Phys & Chem, Beijing 100190, Peoples R China 2.RIKEN, Ctr Sustainable Resource Sci, Enzyme Res Team, Biomass Engn Program Cooperat Div, 2-1 Hirosawa, Wako, Saitama 3510198, Japan 3.Qingdao Univ Sci & Technol, Sch Polymer Sci & Engn, Qingdao 266042, Peoples R China
推荐引用方式 GB/T 7714	Ma, Yinan,Li, Zhibo,Numata, Keiji. Synthetic Short Peptides for Rapid Fabrication of Monolayer Cell Sheets[J]. ACS BIOMATERIALS-SCIENCE & ENGINEERING,2016,2(4):697-706.
APA	Ma, Yinan,Li, Zhibo,&Numata, Keiji.(2016).Synthetic Short Peptides for Rapid Fabrication of Monolayer Cell Sheets.ACS BIOMATERIALS-SCIENCE & ENGINEERING,2(4),697-706.
MLA	Ma, Yinan,et al."Synthetic Short Peptides for Rapid Fabrication of Monolayer Cell Sheets".ACS BIOMATERIALS-SCIENCE & ENGINEERING 2.4(2016):697-706.