Green synthesis and evaluation of isoquercitrin imprinted polymers for class-selective separation and purification of flavonol glycosides

CORC > 新疆理化技术研究所 > 中国科学院新疆理化技术研究所 > 省部共建新疆特有药用资源利用重点实验室

	Green synthesis and evaluation of isoquercitrin imprinted polymers for class-selective separation and purification of flavonol glycosides
	Li, XJ (Li, Xiang-Jie); Chen, XX (Chen, Xiu-Xiu); Sun, GY (Sun, Guan-Yin); Zhao, YX (Zhao, Yong Xin); Liu, ZS (Liu, Zhao-Sheng); Aisa, HA (Aisa, Haji Akber)
刊名	ANALYTICAL METHODS
	2015
卷号	7 期号:11 页码:4717-4724
中文摘要	A method for solid-phase extraction (SPE) of isoquercitrin (ISO) from natural plant extracts was proposed based on molecularly imprinted polymers (MIPs). The efforts in the present work aim at the emphasis on the topic of "green" chemistry, i.e., the use of green solvent and ionic liquid with a high percentage (63.2-69.3%) in the total volume of porogenic solvent. For the preparation of ISO-MIP monoliths, 4-vinylpyridine was used as the functional monomer, and ethylene glycol dimethacrylate was used as the cross-linking monomer, using a mixture of 1-butyl-3-methylimidazoliumtetrafluoroborate (ionic liquid)-N'N-dimethylformamide (DMF)-dimethyl sulfoxide as a porogen. It was found that the type of functional monomer, the ratio of template to functional monomer, the crosslinking degree, and the level of DMF, and the composition of the mobile phase greatly affected the retention of the template and performance of molecular recognition. The optimal MIPs were used as solid-phase extraction (SPE) sorbents for purification of ISO, hyperoside, and astragalin and a SPE protocol was optimised for the type of loading solvent, amount of MIPs, and washing and elution solvents. It was found that the most suitable solvents for loading, washing and elution steps were methanol-water (70 : 30, v/v), methanol-water (20 : 80, v/v) and acetonitrile-water (30 : 70, v/v), respectively. The highest recovery rate of ISO, hyperoside, and astragalin was 87.78%, 93.26% and 83.25%, respectively, from the crude extract of cotton flowers.
收录类别	SCI
资助信息	High Technology Research and Development Program of Xinjiang
内容类型	期刊论文
源URL	[http://ir.xjipc.cas.cn/handle/365002/4535]
专题	新疆理化技术研究所_省部共建新疆特有药用资源利用重点实验室新疆理化技术研究所_资源化学研究室
作者单位	Chinese Acad Sci, Xinjiang Tech Inst Phys & Chem, Urumqi 830011, Xinjiang, Peoples R China;Chinese Acad Sci, Xinjiang Tech Inst Phys & Chem, State Key Lab Basis Xinjiang Indigenous Med Plant, Urumqi 830011, Xinjiang, Peoples R China
推荐引用方式 GB/T 7714	Li, XJ ,Chen, XX ,Sun, GY ,et al. Green synthesis and evaluation of isoquercitrin imprinted polymers for class-selective separation and purification of flavonol glycosides[J]. ANALYTICAL METHODS,2015,7(11):4717-4724.
APA	Li, XJ ,Chen, XX ,Sun, GY ,Zhao, YX ,Liu, ZS ,&Aisa, HA .(2015).Green synthesis and evaluation of isoquercitrin imprinted polymers for class-selective separation and purification of flavonol glycosides.ANALYTICAL METHODS,7(11),4717-4724.
MLA	Li, XJ ,et al."Green synthesis and evaluation of isoquercitrin imprinted polymers for class-selective separation and purification of flavonol glycosides".ANALYTICAL METHODS 7.11(2015):4717-4724.