The alternative respiratory pathway is involved in brassinosteroid-induced environmental stress tolerance in Nicotiana benthamiana

	The alternative respiratory pathway is involved in brassinosteroid-induced environmental stress tolerance in Nicotiana benthamiana
	Deng, Xing-Guang; Zhu, Tong; Zhang, Da-Wei; Lin, Hong-Hui
刊名	JOURNAL OF EXPERIMENTAL BOTANY
	2015
卷号	66 期号:20 页码:6219-6232
关键词	TOBACCO-MOSAIC-VIRUS HYDROGEN-PEROXIDE GENE-EXPRESSION SALICYLIC-ACID ARABIDOPSIS-THALIANA PLANT-MITOCHONDRIA OXIDATIVE STRESS CUCUMIS-SATIVUS H2O2 PRODUCTION BIOTIC STRESS
通讯作者	Lin, HH (reprint author), Sichuan Univ, Coll Life Sci, Key Lab Bioresource & Ecoenvironm, State Key Lab Hydraul & Mt River Engn,Minist Educ, Chengdu 610064, Peoples R China.
产权排序	2
合作状况	国内
英文摘要	Brassinosteroids (BRs), plant steroid hormones, play essential roles in modulating cell elongation, vascular differentiation, senescence, and stress responses. However, the mechanisms by which BRs regulate plant mitochondria and resistance to abiotic stress remain largely unclear. Mitochondrial alternative oxidase (AOX) is involved in the plant response to a variety of environmental stresses. In this report, the role of AOX in BR-induced tolerance against cold, polyethylene glycol (PEG), and high-light stresses was investigated. Exogenous applied brassinolide (BL, the most active BR) induced, while brassinazole (BRZ, a BR biosynthesis inhibitor) reduced alternative respiration and AOX1 expression in Nicotiana benthamiana. Chemical scavenging of H2O2 and virus-induced gene silencing (VIGS) of NbRBOHB compromised the BR-induced alternative respiratory pathway, and this result was further confirmed by NbAOX1 promoter analysis. Furthermore, inhibition of AOX activity by chemical treatment or a VIGS-based approach decreased plant resistance to environmental stresses and compromised BR-induced stress tolerance. Taken together, our results indicate that BR-induced AOX capability might contribute to the avoidance of superfluous reactive oxygen species accumulation and the protection of photosystems under stress conditions in N. benthamiana.
学科主题	Plant Sciences
类目[WOS]	Plant Sciences
语种	英语
内容类型	期刊论文
源URL	[http://210.75.237.14/handle/351003/27493]
专题	成都生物研究所_生态研究
推荐引用方式 GB/T 7714	Deng, Xing-Guang,Zhu, Tong,Zhang, Da-Wei,et al. The alternative respiratory pathway is involved in brassinosteroid-induced environmental stress tolerance in Nicotiana benthamiana[J]. JOURNAL OF EXPERIMENTAL BOTANY,2015,66(20):6219-6232.
APA	Deng, Xing-Guang,Zhu, Tong,Zhang, Da-Wei,&Lin, Hong-Hui.(2015).The alternative respiratory pathway is involved in brassinosteroid-induced environmental stress tolerance in Nicotiana benthamiana.JOURNAL OF EXPERIMENTAL BOTANY,66(20),6219-6232.
MLA	Deng, Xing-Guang,et al."The alternative respiratory pathway is involved in brassinosteroid-induced environmental stress tolerance in Nicotiana benthamiana".JOURNAL OF EXPERIMENTAL BOTANY 66.20(2015):6219-6232.