Industrial-scale culturing of the crude oil-degrading marine Acinetobacter sp. strain HC8-3S

CORC > 烟台海岸带研究所 > 中国科学院烟台海岸带研究所 > 海岸带生物学与生物资源利用重点实验室 > 海岸带生物学与生物资源利用所重点实验室

	Industrial-scale culturing of the crude oil-degrading marine Acinetobacter sp. strain HC8-3S
	Liu, Yuhua 1,2; Hu, Xiaoke1 ; Liu, Hui 3
刊名	International Biodeterioration and Biodegradation
	2016-02-01
卷号	107 页码:56-61
关键词	Crude oil-Bacteria Biodegradation Bioremediation Fermentation Gas chromatography Hydrocarbons Microbiology Petroleum chemistry Temperature Acinetobacter sp Biodegradation efficiency Biodegradation process Contaminated environment Degrading bacteria Industrial scale Long-term storage Petroleum hydrocarbons
ISSN号	0964-8305
通讯作者	Hu, Xiaoke (xkhu@yic.ac.cn)
产权排序	(1) Key Laboratory of Coastal Biology and Bioresource Utilization, Yantai Institute of Coastal Zone Research, Chinese Academy of Sciences, China; (2) University of Chinese Academy of Sciences, Beijing, China; (3) Diyuan Biological Technology Co., Ltd., Yantai, China
英文摘要	The marine bacterium Acinetobacter sp. strain HC8-3S, capable of degrading petroleum hydrocarbons, was previously shown to be applicable for bioremediation. Here, we evaluated the performance of the strain during industrial-scale fermentation, long-term storage, and biodegradation processes. Fermentation parameters were optimized for industrial-scale culturing using lower salinity and fewer inorganic salts in the culture medium. The shelf-life of the fermentation broth was evaluated in different storage conditions. Biodegradation efficiency of the strain was evaluated using gas chromatography. Results indicated that the optimum conditions for one-ton scale fermentation were 30 °C, pH 7.4-7.8, and rotation speed 90 rpm; the cell density reached 3.6 ± 1.9 × 1010CFU ml-1after 12 h. The low temperature can preserved fermentation broth for longer time. When the storage temperature was down to 4 °C from room temperature, half-life of strain HC8-3S extended from 9 days to 34 days. The biodegradation rate of the saturated hydrocarbon fraction of crude oil was 94% after treatment with the strain for 5 days. The results indicate that Acinetobacter sp. strain HC8-3S can be enriched efficiently on a large scale, making it a potentially useful industrial strain. This crude oil degradative capability of the strain HC8-3S provides possible application for the clean-up of crude oil-contaminated environment.
研究领域[WOS]	Environmental Impact and Protection ; Biotechnology ; 6Microbiology ; Petroleum Deposits ; Petroleum Refining, General ; Thermodynamics ; Chemical Operations ; Organic Compounds
语种	英语
WOS记录号	WOS:25
内容类型	期刊论文
源URL	[http://ir.yic.ac.cn/handle/133337/17505]
专题	烟台海岸带研究所_海岸带生物学与生物资源利用所重点实验室
作者单位	1.Key Laboratory of Coastal Biology and Bioresource Utilization, Yantai Institute of Coastal Zone Research, Chinese Academy of Sciences 2.University of Chinese Academy of Sciences 3.Diyuan Biological Technology Co., Ltd
推荐引用方式 GB/T 7714	Liu, Yuhua,Hu, Xiaoke,Liu, Hui. Industrial-scale culturing of the crude oil-degrading marine Acinetobacter sp. strain HC8-3S[J]. International Biodeterioration and Biodegradation,2016,107:56-61.
APA	Liu, Yuhua,Hu, Xiaoke,&Liu, Hui.(2016).Industrial-scale culturing of the crude oil-degrading marine Acinetobacter sp. strain HC8-3S.International Biodeterioration and Biodegradation,107,56-61.
MLA	Liu, Yuhua,et al."Industrial-scale culturing of the crude oil-degrading marine Acinetobacter sp. strain HC8-3S".International Biodeterioration and Biodegradation 107(2016):56-61.