Goldfish Leptin-AI and Leptin-AII: Function and Central Mechanism in Feeding Control

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	Goldfish Leptin-AI and Leptin-AII: Function and Central Mechanism in Feeding Control
	Yan, Ai-Fen; Chen, Ting; Chen, Shuang; Ren, Chun-Hua; Hu, Chao-Qun; Cai, Yi-Ming; Liu, Fang; Tang, Dong-Sheng
刊名	INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
	2016
卷号	17 期号:6 页码:783
关键词	leptin goldfish methylotrophic yeast feeding control gene regulation appetite regulators
通讯作者	Chen, T (reprint author), Chinese Acad Sci, CAS Key Lab Trop Marine Bioresources & Ecol LMB, Guangdong Prov Key Lab Appl Marine Biol LAMB, South China Sea Inst Oceanol, Guangzhou 510301, Guangdong, Peoples R China. ; Chen, T (reprint author), South China Sea Bioresource Exploitat & Utilizat, Guangzhou 510275, Guangdong, Peoples R China.
中文摘要	In mammals, leptin is a peripheral satiety factor that inhibits feeding by regulating a variety of appetite-related hormones in the brain. However, most of the previous studies examining leptin in fish feeding were performed with mammalian leptins, which share very low sequence homologies with fish leptins. To elucidate the function and mechanism of endogenous fish leptins in feeding regulation, recombinant goldfish leptin-AI and leptin-AII were expressed in methylotrophic yeast and purified by immobilized metal ion affinity chromatography (IMAC). By intraperitoneal (IP) injection, both leptin-AI and leptin-AII were shown to inhibit the feeding behavior and to reduce the food consumption of goldfish in 2 h. In addition, co-treatment of leptin-AI or leptin-AII could block the feeding behavior and reduce the food consumption induced by neuropeptide Y (NPY) injection. High levels of leptin receptor (lepR) mRNA were detected in the hypothalamus, telencephalon, optic tectum and cerebellum of the goldfish brain. The appetite inhibitory effects of leptins were mediated by downregulating the mRNA levels of orexigenic NPY, agouti-related peptide (AgRP) and orexin and upregulating the mRNA levels of anorexigenic cocaine-amphetamine-regulated transcript (CART), cholecystokinin (CCK), melanin-concentrating hormone (MCH) and proopiomelanocortin (POMC) in different areas of the goldfish brain. Our study, as a whole, provides new insights into the functions and mechanisms of leptins in appetite control in a fish model.
学科主题	Biochemistry & Molecular Biology; Chemistry
内容类型	期刊论文
源URL	[http://ir.scsio.ac.cn/handle/344004/15402]
专题	南海海洋研究所_中科院海洋生物资源可持续利用重点实验室
推荐引用方式 GB/T 7714	Yan, Ai-Fen,Chen, Ting,Chen, Shuang,et al. Goldfish Leptin-AI and Leptin-AII: Function and Central Mechanism in Feeding Control[J]. INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES,2016,17(6):783.
APA	Yan, Ai-Fen.,Chen, Ting.,Chen, Shuang.,Ren, Chun-Hua.,Hu, Chao-Qun.,...&Tang, Dong-Sheng.(2016).Goldfish Leptin-AI and Leptin-AII: Function and Central Mechanism in Feeding Control.INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES,17(6),783.
MLA	Yan, Ai-Fen,et al."Goldfish Leptin-AI and Leptin-AII: Function and Central Mechanism in Feeding Control".INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES 17.6(2016):783.