Tetraodon nigroviridis: A model of Vibrio parahaemolyticus infection

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	Tetraodon nigroviridis: A model of Vibrio parahaemolyticus infection
	Peng, Wan; Shi, Yu; Li, Gao-Fei; He, Liang-Ge; Liang, Yao-Si; Zhang, Yong; Zhou, Li-Bin; Lin, Hao-Ran; Lu, Dan-Qi
刊名	FISH & SHELLFISH IMMUNOLOGY
	2016
卷号	56 页码:388-396
关键词	Vibrio parahaemolyticus Tetraodon nigroviridis Liver miR-29b Pattern recognition receptors
通讯作者	Lu, DQ (reprint author), Sun Yat Sen Univ, State Key Lab Biocontrol, Inst Aquat Econ Anim, Coll Life Sci, Guangzhou 510275, Guangdong, Peoples R China. ; Lu, DQ (reprint author), Sun Yat Sen Univ, Guangdong Prov Key Lab Aquat Econ Anim, Coll Life Sci, Guangzhou 510275, Guangdong, Peoples R China.
英文摘要	Vibriosis is the most common bacterial diseases and brings great economic loss on aquaculture. Vibrio parahaemolyticus (V. parahaemolyticus), a gram-negative bacterium, has been identified as one main pathogens of Vibriosis. The pathogenic mechanism of V. parahaemolyticus is not entirely clear now. In our study, a model of V. parahaemolyticus infection of green-spotted puffer fish (Tetraodon nigroviridis) was established. T. nigroviridis were injected intraperitoneally (i.p.) with 200 mu L of V. parahaemolyticus (8 x 10(10) CFU/mL). V parahaemolyticus infection caused 64% mortality and infected some organs of T. nigroviridis. Histopathology studies revealed V. parahaemolyticus infection induced tissue structural changes, including adipose hollow space in the liver. Immunohistochemistry showed V. parahaemolyticus were present in infected tissue such as liver, head kidney and spleen. In livers of T nigroviridis infected by V. parahaemolyticus, the alkaline phosphatases (ALP) activity first gradually increased and then backed to normal level, a trend that was on the contrary to the expression profile of the miR-29b. Quantitative real-time PCR analysis showed that the expression level of TLR1, TLR2, TLR5, TLR9, TLR21, NOD1, NOD2 and IL-6 in response to V. parahaemolyticus infection decreased compared to that of non-infected fish. The establishment of the T. nigroviridis model of V. parahaemolyticus infection further confirmed V. parahaemolyticus spreads through the blood circulation system primary as an extracellular pathogen. Meanwhile, liver is an important target organ when infected by V. parahaemolyticus. miR-29b in liver was involved in the progress of liver steatosis during V. parahaemolyticus infection. Moreover, V. parahaemolyticus infection in vivo may have an effect of immunosuppression on host. (C) 2016 Elsevier Ltd. All rights reserved.
学科主题	Fisheries; Immunology; Marine & Freshwater Biology; Veterinary Sciences
内容类型	期刊论文
源URL	[http://ir.scsio.ac.cn/handle/344004/15300]
专题	南海海洋研究所_中科院海洋生物资源可持续利用重点实验室
推荐引用方式 GB/T 7714	Peng, Wan,Shi, Yu,Li, Gao-Fei,et al. Tetraodon nigroviridis: A model of Vibrio parahaemolyticus infection[J]. FISH & SHELLFISH IMMUNOLOGY,2016,56:388-396.
APA	Peng, Wan.,Shi, Yu.,Li, Gao-Fei.,He, Liang-Ge.,Liang, Yao-Si.,...&Lu, Dan-Qi.(2016).Tetraodon nigroviridis: A model of Vibrio parahaemolyticus infection.FISH & SHELLFISH IMMUNOLOGY,56,388-396.
MLA	Peng, Wan,et al."Tetraodon nigroviridis: A model of Vibrio parahaemolyticus infection".FISH & SHELLFISH IMMUNOLOGY 56(2016):388-396.