Real-time Imaging of Rabies Virus Entry into Living Vero cells

CORC > 南海海洋研究所 > 中国科学院南海海洋研究所 > 中科院海洋生物资源可持续利用重点实验室

	Real-time Imaging of Rabies Virus Entry into Living Vero cells
	Xu, Haijiao 1; Hao, Xian 1; Wang, Shaowen 1; Wang, Zhiyong 1; Cai, Mingjun 1; Jiang, Junguang 1; Qin, Qiwei 1; Zhang, Maolin 1; Wang, Hongda 1
刊名	SCIENTIFIC REPORTS
	2015
卷号	5 页码:11753-
通讯作者	zhrei98@163.com ; hdwang@ciac.ac.cn
英文摘要	Understanding the mechanism of rabies virus (RABV) infection is vital for prevention and therapy of virulent rabies. However, the infection mechanism remains largely uncharacterized due to the limited methods and viral models. Herein, we utilized a powerful single-virus tracking technique to dynamically and globally visualize the infection process of the live attenuated rabies vaccine strain-SRV9 in living Vero cells. Firstly, it was found that the actin-enriched filopodia is in favor of virus reaching to the cell body. Furthermore, by carrying out drug perturbation experiments, we confirmed that RABV internalization into Vero cells proceeds via classical dynamin-dependent clathrin-mediated endocytosis with requirement for intact actin, but caveolae-dependent endocytosis is not involved. Then, our real-time imaging results unambiguously uncover the characteristics of viral internalization and cellular transport dynamics. In addition, our results directly and quantitatively reveal that the intracellular motility of internalized RABV particles is largely microtubule-dependent. Collectively, our work is crucial for understanding the initial steps of RABV infection, and elucidating the mechanisms of post-infection. Significantly, the results provide profound insight into development of novel and effective antiviral targets.
学科主题	Science & Technology - Other Topics
原文出处	2045-2322
内容类型	期刊论文
源URL	[http://ir.scsio.ac.cn/handle/344004/14804]
专题	南海海洋研究所_中科院海洋生物资源可持续利用重点实验室
作者单位	1.[Xu, Haijiao 2.Hao, Xian 3.Wang, Zhiyong 4.Cai, Mingjun 5.Jiang, Junguang 6.Wang, Hongda] Chinese Acad Sci, Changchun Inst Appl Chem, State Key Lab Electroanalyt Chem, Changchun 130022, Jilin, Peoples R China 7.[Wang, Shaowen 8.Qin, Qiwei] Chinese Acad Sci, South China Sea Inst Oceanol, Key Lab Trop Marine Bioresources & Ecol, Guangzhou, Guangdong, Peoples R China 9.[Wang, Shaowen 10.Qin, Qiwei
推荐引用方式 GB/T 7714	Xu, Haijiao,Hao, Xian,Wang, Shaowen,et al. Real-time Imaging of Rabies Virus Entry into Living Vero cells[J]. SCIENTIFIC REPORTS,2015,5:11753-.
APA	Xu, Haijiao.,Hao, Xian.,Wang, Shaowen.,Wang, Zhiyong.,Cai, Mingjun.,...&Wang, Hongda.(2015).Real-time Imaging of Rabies Virus Entry into Living Vero cells.SCIENTIFIC REPORTS,5,11753-.
MLA	Xu, Haijiao,et al."Real-time Imaging of Rabies Virus Entry into Living Vero cells".SCIENTIFIC REPORTS 5(2015):11753-.