Genomic insights into the taxonomic status of the Bacillus cereus group

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	Genomic insights into the taxonomic status of the Bacillus cereus group
	Liu, Yang 1; Lai, Qiliang 1; Goeker, Markus 1; Meier-Kolthoff, Jan P.1; Wang, Meng 1; Sun, Yamin 1; Wang, Lei 1; Shao, Zongze 1
刊名	SCIENTIFIC REPORTS
	2015
卷号	5 页码:14082-
通讯作者	shaozz@163.com
英文摘要	The identification and phylogenetic relationships of bacteria within the Bacillus cereus group are controversial. This study aimed at determining the taxonomic affiliations of these strains using the whole-genome sequence-based Genome BLAST Distance Phylogeny (GBDP) approach. The GBDP analysis clearly separated 224 strains into 30 clusters, representing eleven known, partially merged species and accordingly 19-20 putative novel species. Additionally, 16S rRNA gene analysis, a novel variant of multi-locus sequence analysis (nMLSA) and screening of virulence genes were performed. The 16S rRNA gene sequence was not sufficient to differentiate the bacteria within this group due to its high conservation. The nMLSA results were consistent with GBDP. Moreover, a fast typing method was proposed using the pycA gene, and where necessary, the ccpA gene. The pXO plasmids and cry genes were widely distributed, suggesting little correlation with the phylogenetic positions of the host bacteria. This might explain why classifications based on virulence characteristics proved unsatisfactory in the past. In summary, this is the first large-scale and systematic study of the taxonomic status of the bacteria within the B. cereus group using whole-genome sequences, and is likely to contribute to further insights into their pathogenicity, phylogeny and adaptation to diverse environments.
学科主题	Science & Technology - Other Topics
原文出处	2045-2322
内容类型	期刊论文
源URL	[http://ir.scsio.ac.cn/handle/344004/14772]
专题	南海海洋研究所_中科院海洋生物资源可持续利用重点实验室
作者单位	1.[Liu, Yang 2.Lai, Qiliang 3.Shao, Zongze] State Key Lab Breeding Base Marine Genet Resource, Xiamen 361005, Peoples R China 4.[Liu, Yang 5.Lai, Qiliang 6.Shao, Zongze] SOA, Ins Oceanog 3, Key Lab Marine Genet Resources, Xiamen 361005, Peoples R China 7.[Liu, Yang 8.Lai, Qiliang 9.Shao, Zongze] South China Sea Bio Resource Exploitat & Utilizat, Xiamen 361005, Peoples R China 10.[Liu, Yang
推荐引用方式 GB/T 7714	Liu, Yang,Lai, Qiliang,Goeker, Markus,et al. Genomic insights into the taxonomic status of the Bacillus cereus group[J]. SCIENTIFIC REPORTS,2015,5:14082-.
APA	Liu, Yang.,Lai, Qiliang.,Goeker, Markus.,Meier-Kolthoff, Jan P..,Wang, Meng.,...&Shao, Zongze.(2015).Genomic insights into the taxonomic status of the Bacillus cereus group.SCIENTIFIC REPORTS,5,14082-.
MLA	Liu, Yang,et al."Genomic insights into the taxonomic status of the Bacillus cereus group".SCIENTIFIC REPORTS 5(2015):14082-.