Expression in Pichia pastoris and characterization of Rhizomucor miehei lipases containing a new propeptide region

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	Expression in Pichia pastoris and characterization of Rhizomucor miehei lipases containing a new propeptide region
	Wang, Zhiyuan; Lv, Pengmei; Luo, Wen; Yuan, Zhenhong; He, Dong
刊名	journal of general and applied microbiology
	2016
卷号	62 期号:1 页码:25-30
关键词	enzyme property intramolecular chaperone propeptide replacing protein folding Rhizomucor miehei lipases
英文摘要	a large number of propeptide regions from various proteins have been identified which function as intramolecular chaperones and assist the folding of the respective functional domains. the same polypeptide can fold into an altered conformation because of a mutated intramolecular chaperone and can maintain the "memory" of the folding process (new physicochemical properties). two new kinds of rhizomucor miehei lipase (rml) were constructed by replacing its propeptide region with that from either rhizopus chinensis lipase (rcl) or rhizopus oryzae lipase (rol). the enzymatic properties were also analyzed and compared between wild-type rml and the mutants. the results indicated that the same polypeptide can fold into different conformations because of changes in the propeptide region.
WOS标题词	science & technology ; life sciences & biomedicine
类目[WOS]	biotechnology & applied microbiology ; microbiology
研究领域[WOS]	biotechnology & applied microbiology ; microbiology
关键词[WOS]	intramolecular chaperones ; pro-sequence ; enhanced activity ; escherichia-coli ; precursor ; protease
收录类别	SCI
语种	英语
WOS记录号	WOS:000374814700005
内容类型	期刊论文
源URL	[http://ir.giec.ac.cn/handle/344007/11276]
专题	中国科学院广州能源研究所
作者单位	Chinese Acad Sci, Key Lab Renewable Energy & Gas Hydrate, Guangzhou Inst Energy Convers, 2 Nengyuan RD, Guangzhou 510640, Guangdong, Peoples R China
推荐引用方式 GB/T 7714	Wang, Zhiyuan,Lv, Pengmei,Luo, Wen,et al. Expression in Pichia pastoris and characterization of Rhizomucor miehei lipases containing a new propeptide region[J]. journal of general and applied microbiology,2016,62(1):25-30.
APA	Wang, Zhiyuan,Lv, Pengmei,Luo, Wen,Yuan, Zhenhong,&He, Dong.(2016).Expression in Pichia pastoris and characterization of Rhizomucor miehei lipases containing a new propeptide region.journal of general and applied microbiology,62(1),25-30.
MLA	Wang, Zhiyuan,et al."Expression in Pichia pastoris and characterization of Rhizomucor miehei lipases containing a new propeptide region".journal of general and applied microbiology 62.1(2016):25-30.