Systematic evaluation of sericin protein as a substitute for fetal bovine serum in cell culture

	Systematic evaluation of sericin protein as a substitute for fetal bovine serum in cell culture
	Liu LY 1,2; Wang JH 1; Dong Y[]3; Wang W[]1,3; Duan SC 3; Chen L 1,2; Xiang H[*]1,4
刊名	SCIENTIFIC REPORTS
	2016
卷号	6 期号:X 页码:e31516
通讯作者	xiangh@mail.kiz.ac.cn
合作状况	其它
英文摘要	Fetal bovine serum (FBS) shows obvious deficiencies in cell culture, such as low batch to batch consistency, adventitious biological contaminant risk, and high cost, which severely limit the development of the cell culture industry. Sericin protein derived from the silkworm cocoon has become increasingly popular due to its diverse and beneficial cell culture characteristics. However, systematic evaluation of sericin as a substitute for FBS in cell culture medium remains limited. In this study, we conducted cellular morphological, physiological, and transcriptomic evaluation on three widely used mammalian cells. Compared with cells cultured in the control, those cultured in sericin-substitute medium showed similar cellular morphology, similar or higher cellular overall survival, lower population doubling time (PDT), and a higher percentage of S-phase with similar G2/G1 ratio, indicating comparable or better cell growth and proliferation. At the transcriptomic level, differentially expressed genes between cells in the two media were mainly enriched in function and biological processes related to cell growth and proliferation, reflecting that genes were activated to facilitate cell growth and proliferation. The results of this study suggest that cells cultured in sericin-substituted medium perform as well as, or even better than, those cultured in FBS-containing medium.
收录类别	SCI
资助信息	This work was supported by a 973 Program grant (no. 2013CB835200), two National Natural Science Foundation of China grants (no. 31371286 and no. 31123005).
语种	英语
内容类型	期刊论文
源URL	[http://159.226.149.26:8080/handle/152453/10392]
专题	昆明动物研究所_基因起源组昆明动物研究所_遗传资源与进化国家重点实验室昆明动物研究所_保护遗传学(细胞库)
作者单位	1.State Key Laboratory of Genetic Resources and Evolution, Kunming Institute of Zoology, Chinese Academy of Sciences, Kunming 650223, China 2.Kunming College of Life Science, University of Chinese Academy of Sciences, Kunming 650223, China 3.Kunming University of Science and Technology, 727 South Jingming Road, Chenggong District, Kunming 650500, China. 4.South China Normal University, Guangzhou, 510631, China
推荐引用方式 GB/T 7714	Liu LY,Wang JH,Dong Y[*],et al. Systematic evaluation of sericin protein as a substitute for fetal bovine serum in cell culture[J]. SCIENTIFIC REPORTS,2016,6(X):e31516.
APA	Liu LY.,Wang JH.,Dong Y[].,Wang W[].,Duan SC.,...&Xiang H[*].(2016).Systematic evaluation of sericin protein as a substitute for fetal bovine serum in cell culture.SCIENTIFIC REPORTS,6(X),e31516.
MLA	Liu LY,et al."Systematic evaluation of sericin protein as a substitute for fetal bovine serum in cell culture".SCIENTIFIC REPORTS 6.X(2016):e31516.