A large stokes-shifted fluorescent dye synthesized as a new probe for the determination of protein

CORC > 化学研究所 > 中国科学院化学研究所 > 分子动态与稳态结构实验室

	A large stokes-shifted fluorescent dye synthesized as a new probe for the determination of protein
	Lin, Dayong 1; Fei, Xuening 1,2; Li, Ran 2; Gu, Yingchun 2,3; Tang, Yalin 3; Zhou, Jianguo 2; Zhang, Baolian 2
刊名	JOURNAL OF FLUORESCENCE
	2016-07-01
卷号	26 期号:4 页码:1511-1520
关键词	Spectra properties Fluorescent probe Protein Specific
英文摘要	A novel fluorescent dye, 1-(2-hydroxyethyl)-4-((E)-2-(3-benzothiazol-2yl-9-ethyl-carbazole-3yl)vinyl) pyridinium bromide, was synthesized for determination of protein and its structure was characterized by H-1 NMR. Photophysics of the new probe in different solvents has been delineated in this paper, the new fluorescent molecular dye exhibited a large stokes-shifted and fluorescence quantum yields in organic solvent. The photostability and thermostability of the new dye were also studied and the results suggested the stable was excellent. The interactions of the dye with bovine serum albumin (BSA) , Human serumal bumin (HSA) and calf thymus deoxyribonucleic acid (ctDNA) were studied by fluorescence and absorption spectroscopy. The binding constant for BSA, HSA and DNA were calculated to be 8.91 x 10(7), 1.86 x 10(6) and 2.9 x 10(4), respectively. The experimental results indicated a potential value of the new dye for biomarker.
收录类别	SCI
语种	英语
内容类型	期刊论文
源URL	[http://ir.iccas.ac.cn/handle/121111/35370]
专题	化学研究所_分子动态与稳态结构实验室
作者单位	1.Tianjin Univ, Sch Envirnmental Sci & Engn, 92 Weijin Rd, Tianjin 300072, Peoples R China 2.Tianjin Chengjian Univ, Sch Sci, 26 Jinjing Rd, Tianjin 300384, Peoples R China 3.Chinese Acad Sci ICCAS, Inst Chem, Zhongguancun North First St 2, Beijing 100190, Peoples R China
推荐引用方式 GB/T 7714	Lin, Dayong,Fei, Xuening,Li, Ran,et al. A large stokes-shifted fluorescent dye synthesized as a new probe for the determination of protein[J]. JOURNAL OF FLUORESCENCE,2016,26(4):1511-1520.
APA	Lin, Dayong.,Fei, Xuening.,Li, Ran.,Gu, Yingchun.,Tang, Yalin.,...&Zhang, Baolian.(2016).A large stokes-shifted fluorescent dye synthesized as a new probe for the determination of protein.JOURNAL OF FLUORESCENCE,26(4),1511-1520.
MLA	Lin, Dayong,et al."A large stokes-shifted fluorescent dye synthesized as a new probe for the determination of protein".JOURNAL OF FLUORESCENCE 26.4(2016):1511-1520.