The role of Arabidopsis Actin-Related Protein 3 in amyloplast sedimentation and polar auxin transport in root gravitropism

	The role of Arabidopsis Actin-Related Protein 3 in amyloplast sedimentation and polar auxin transport in root gravitropism
	Zou JJ; Zheng ZY(郑中玉); Xue S; Li HH(李瀚海); Wang YR(王育人); Le J; Li HH(李瀚海); Wang YR(王育人); Zheng ZY(郑中玉)
刊名	JOURNAL OF EXPERIMENTAL BOTANY
	2016
通讯作者邮箱	yurenwang@imech.ac.cn ; lejie@ibcas.ac.cn
卷号	67 期号:18 页码:5325-5337
关键词	Amyloplast arabidopsis ARP2/3 auxin gravitropism PINs
ISSN号	0022-0957
通讯作者	Le, J (reprint author), Chinese Acad Sci, Inst Bot, CAS Ctr Excellence Mol Plant Sci, Key Lab Plant Mol Physiol, Beijing 100093, Peoples R China. ; Wang, YR (reprint author), Chinese Acad Sci, Inst Mech, Key Lab Micrograv, Beijing 100190, Peoples R China.
产权排序	[Zou, Jun-Jie; Le, Jie] Chinese Acad Sci, Inst Bot, CAS Ctr Excellence Mol Plant Sci, Key Lab Plant Mol Physiol, Beijing 100093, Peoples R China; [Zheng, Zhong-Yu; Li, Han-Hai; Wang, Yu-Ren] Chinese Acad Sci, Inst Mech, Key Lab Micrograv, Beijing 100190, Peoples R China; [Xue, Shan] Univ Chinese Acad Sci, 19A Yuquan Rd, Beijing 100049, Peoples R China
中文摘要	Arabidopsis actin-related protein ARP3 plays a role in amyloplast sedimentation and polar auxin redistribution during root gravitropism.Gravitropism is vital for shaping directional plant growth in response to the forces of gravity. Signals perceived in the gravity-sensing cells can be converted into biochemical signals and transmitted. Sedimentation of amyloplasts in the columella cells triggers asymmetric auxin redistribution in root tips, leading to downward root growth. The actin cytoskeleton is thought to play an important role in root gravitropism, although the molecular mechanism has not been resolved. DISTORTED1 (DIS1) encodes the ARP3 subunit of the Arabidopsis Actin-Related Protein 2/3 (ARP2/3) complex, and the ARP3/DIS1 mutant dis1-1 showed delayed root curvature after gravity stimulation. Microrheological analysis revealed that the high apparent viscosity within dis1-1 central columella cells is closely associated with abnormal movement trajectories of amyloplasts. Analysis using a sensitive auxin input reporter DII-VENUS showed that asymmetric auxin redistribution was reduced in the root tips of dis1-1, and the actin-disrupting drug Latrunculin B increased the asymmetric auxin redistribution. An uptake assay using the membrane-selective dye FM4-64 indicated that endocytosis was decelerated in dis1-1 root epidermal cells. Treatment and wash-out with Brefeldin A, which inhibits protein transport from the endoplasmic reticulum to the Golgi apparatus, showed that cycling of the auxin-transporter PIN-FORMED (PIN) proteins to the plasma membrane was also suppressed in dis1-1 roots. The results reveal that ARP3/DIS1 acts in root gravitropism by affecting amyloplast sedimentation and PIN-mediated polar auxin transport through regulation of PIN protein trafficking.
分类号	一类
类目[WOS]	Plant Sciences
研究领域[WOS]	Plant Sciences
关键词[WOS]	Amyloplast ; arabidopsis ; ARP2/3 ; auxin ; gravitropism ; PINs
收录类别	SCI ; EI
原文出处	http://dx.doi.org/10.1093/jxb/erw294
语种	英语
WOS记录号	WOS:000386067000006
内容类型	期刊论文
源URL	[http://dspace.imech.ac.cn/handle/311007/59699]
专题	力学研究所_国家微重力实验室
推荐引用方式 GB/T 7714	Zou JJ,Zheng ZY,Xue S,et al. The role of Arabidopsis Actin-Related Protein 3 in amyloplast sedimentation and polar auxin transport in root gravitropism[J]. JOURNAL OF EXPERIMENTAL BOTANY,2016,67(18):5325-5337.
APA	Zou JJ.,郑中玉.,Xue S.,李瀚海.,王育人.,...&Zheng ZY.(2016).The role of Arabidopsis Actin-Related Protein 3 in amyloplast sedimentation and polar auxin transport in root gravitropism.JOURNAL OF EXPERIMENTAL BOTANY,67(18),5325-5337.
MLA	Zou JJ,et al."The role of Arabidopsis Actin-Related Protein 3 in amyloplast sedimentation and polar auxin transport in root gravitropism".JOURNAL OF EXPERIMENTAL BOTANY 67.18(2016):5325-5337.