Critical evaluation of the expression of gastrin-releasing peptide in dorsal root ganglia and spinal cord

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	Critical evaluation of the expression of gastrin-releasing peptide in dorsal root ganglia and spinal cord
	Barry, DM; Li, H; Liu, XY; Shen, KF; Liu, XT; Wu, ZY; Munanairi, A; Chen, XJ; Yin, J; Sun, YG
刊名	MOLECULAR PAIN
	2016
卷号	12 页码:e1111
关键词	BOMBESIN-LIKE IMMUNOREACTIVITY DELTA-OPIOID RECEPTORS CUTANEOUS NERVE-FIBERS SUBSTANCE-P RNA-SEQ ATOPIC-DERMATITIS SENSORY NEURONS ITCH TRANSMISSION PRIMARY AFFERENT GENE-EXPRESSION
通讯作者	Chen, ZF (reprint author), Washington Univ, Sch Med, St Louis, MO USA. ; Li, YQ (reprint author), Fourth Mil Med Univ, Dept Anat, Xian 710032, Peoples R China. ; Li, YQ (reprint author), Fourth Mil Med Univ, KK Leung Brain Res Ctr, Xian 710032, Peoples R China.,deptanat@fmmu.edu.cn ; chenz@wustl.edu
英文摘要	There are substantial disagreements about the expression of gastrin-releasing peptide (GRP) in sensory neurons and whether GRP antibody cross-reacts with substance P (SP). These concerns necessitate a critical revaluation of GRP expression using additional approaches. Here, we show that a widely used GRP antibody specifically recognizes GRP but not SP. In the spinal cord of mice lacking SP (Tac1 KO), the expression of not only GRP but also other peptides, notably neuropeptide Y (NPY), is significantly diminished. We detected Grp mRNA in dorsal root ganglias using reverse transcription polymerase chain reaction, in situ hybridization and RNA-seq. We demonstrated that Grp mRNA and protein are upregulated in dorsal root ganglias, but not in the spinal cord, of mice with chronic itch. Few GRP(+) immunostaining signals were detected in spinal sections following dorsal rhizotomy and GRP(+) cell bodies were not detected in dissociated dorsal horn neurons. Ultrastructural analysis further shows that substantially more GRPergic fibers form synaptic contacts with gastrin releasing peptide receptor-positive (GRPR(+)) neurons than SPergic fibers. Our comprehensive study demonstrates that a majority of GRPergic fibers are of primary afferent origin. A number of factors such as low copy number of Grp transcripts, small percentage of cells expressing Grp, and the use of an eGFP GENSAT transgenic as a surrogate for GRP protein have contributed to the controversy. Optimization of experimental procedures facilitates the specific detection of GRP expression in dorsal root ganglia neurons.
学科主题	Neurosciences
WOS标题词	Neurosciences & Neurology
语种	英语
WOS记录号	WOS:000376718000001
内容类型	期刊论文
源URL	[http://ir.sibs.ac.cn/handle/331001/4030]
专题	上海神经科学研究所_神经所(总)
推荐引用方式 GB/T 7714	Barry, DM,Li, H,Liu, XY,et al. Critical evaluation of the expression of gastrin-releasing peptide in dorsal root ganglia and spinal cord[J]. MOLECULAR PAIN,2016,12:e1111.
APA	Barry, DM.,Li, H.,Liu, XY.,Shen, KF.,Liu, XT.,...&Chen, ZF.(2016).Critical evaluation of the expression of gastrin-releasing peptide in dorsal root ganglia and spinal cord.MOLECULAR PAIN,12,e1111.
MLA	Barry, DM,et al."Critical evaluation of the expression of gastrin-releasing peptide in dorsal root ganglia and spinal cord".MOLECULAR PAIN 12(2016):e1111.