Basal Forebrain Cholinergic Neurons Primarily Contribute to Inhibition of Electroencephalogram Delta Activity; Rather Than Inducing Behavioral Wakefulness in Mice

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	Basal Forebrain Cholinergic Neurons Primarily Contribute to Inhibition of Electroencephalogram Delta Activity; Rather Than Inducing Behavioral Wakefulness in Mice
	Chen, L; Yin, D; Wang, TX; Guo, W; Dong, H; Xu, Q; Luo, YJ; Cherasse, Y; Lazarus, M; Qiu, ZL
刊名	NEUROPSYCHOPHARMACOLOGY
	2016
卷号	41 期号:8 页码:2133-2146
关键词	NUCLEUS BASALIS IN-VITRO SLEEP WAKE MODULATION RECEPTORS ADENOSINE WAKING THALAMUS RATS
通讯作者	Qu, WM ; Huang, ZL (reprint author), Fudan Univ, Sch Basic Med Sci, Dept Pharmacol, Shanghai 200032, Peoples R China.,quweimin@fudan.edu.cn ; huangzl@fudan.edu.cn
英文摘要	The basal forebrain (BF) cholinergic neurons have long been thought to be involved in behavioral wakefulness and cortical activation. However, owing to the heterogeneity of BF neurons and poor selectivity of traditional methods, the precise role of BF cholinergic neurons in regulating the sleep wake cycle remains unclear. We investigated the effects of cell-selective manipulation of BF cholinergic neurons on the sleep wake behavior and electroencephalogram (EEG) power spectrum using the pharmacogenetic technique, the 'designer receptors exclusively activated by designer drugs (DREADD)' approach, and ChAT-IRES-Cre mice. Our results showed that activation of BF cholinergic neurons expressing hM3Dq receptors significantly and lastingly decreased the EEG delta power spectrum, produced low-delta non-rapid eye movement sleep, and slightly increased wakefulness in both light and dark phases, whereas inhibition of BF cholinergic neurons expressing hM4Di receptors significantly increased EEG delta power spectrum and slightly decreased wakefulness. Next, the projections of BF cholinergic neurons were traced by humanized Renilla green fluorescent protein (hrGFP). Abundant and highly dense hrGFP-positive fibers were observed in the secondary motor cortex and cingulate cortex, and sparse hrGFP-positive fibers were observed in the ventrolateral preoptic nucleus, a known sleep-related structure. Finally, we found that activation of BF cholinergic neurons significantly increased c-Fos expression in the secondary motor cortex and cingulate cortex, but decreased c-Fos expression in the ventrolateral preoptic nucleus. Taken together, these findings reveal that the primary function of BF cholinergic neurons is to inhibit EEG delta activity through the activation of cerebral cortex, rather than to induce behavioral wakefulness.
学科主题	Neurosciences; Pharmacology & Pharmacy; Psychiatry
WOS标题词	Neurosciences & Neurology ; Pharmacology & Pharmacy ; Psychiatry
语种	英语
WOS记录号	WOS:000377842800021
内容类型	期刊论文
源URL	[http://ir.sibs.ac.cn/handle/331001/4016]
专题	上海神经科学研究所_神经所(总)
推荐引用方式 GB/T 7714	Chen, L,Yin, D,Wang, TX,et al. Basal Forebrain Cholinergic Neurons Primarily Contribute to Inhibition of Electroencephalogram Delta Activity; Rather Than Inducing Behavioral Wakefulness in Mice[J]. NEUROPSYCHOPHARMACOLOGY,2016,41(8):2133-2146.
APA	Chen, L.,Yin, D.,Wang, TX.,Guo, W.,Dong, H.,...&Huang, ZL.(2016).Basal Forebrain Cholinergic Neurons Primarily Contribute to Inhibition of Electroencephalogram Delta Activity; Rather Than Inducing Behavioral Wakefulness in Mice.NEUROPSYCHOPHARMACOLOGY,41(8),2133-2146.
MLA	Chen, L,et al."Basal Forebrain Cholinergic Neurons Primarily Contribute to Inhibition of Electroencephalogram Delta Activity; Rather Than Inducing Behavioral Wakefulness in Mice".NEUROPSYCHOPHARMACOLOGY 41.8(2016):2133-2146.