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	In vivo vascular imaging with adaptive optics confocal scanning fluorescence microscopy
	Wang, Zhibin1,2,3; Wei, Lin1,2; He, Yi1,2; Li, Xiqi1,2; Shi, Guohua1,2; Zhang, Yudong1,2
	2014
会议名称	Proceedings of SPIE: Twelfth International Conference on Photonics and Imaging in Biology and Medicine, PIBM 2014
会议日期	2014
卷号	9230
页码	92301W
通讯作者	Wang, Zhibin
中文摘要	Adaptive optics is implemented in a confocal scanning fluorescence microscope with wavefront sensorless scheme. Using the image sharpness as the optimization metric, aberration correction is performed to compensate both system- and specimen-induced aberrations by using stochastic parallel gradient descent algorithm based upon Zernike polynomial modes. In vivo vascular imaging of mice ear is completed and the results revealed the improved signal and resolution leading to in substantially enhanced image contrast with aberration correction which allowed us to detect clearer vasculature structures. © 2014 SPIE.
英文摘要	Adaptive optics is implemented in a confocal scanning fluorescence microscope with wavefront sensorless scheme. Using the image sharpness as the optimization metric, aberration correction is performed to compensate both system- and specimen-induced aberrations by using stochastic parallel gradient descent algorithm based upon Zernike polynomial modes. In vivo vascular imaging of mice ear is completed and the results revealed the improved signal and resolution leading to in substantially enhanced image contrast with aberration correction which allowed us to detect clearer vasculature structures. © 2014 SPIE.
收录类别	EI
学科主题	Confocal microscopy - Fluorescence - Fluorescence microscopy - Gradient methods - Photonics - Stochastic systems
语种	英语
ISSN号	16057422
内容类型	会议论文
源URL	[http://ir.ioe.ac.cn/handle/181551/7824]
专题	光电技术研究所_自适应光学技术研究室（八室）
作者单位	1.Key Laboratory on Adaptive Optics, Chinese Academy of Sciences, Chengdu, China 2.Laboratory on Adaptive Optics, Institute of Optics and Electronics, Chinese Academy of Sciences, Chengdu, China 3.Graduate School of Chinese, Academy of Sciences, Beijing, China
推荐引用方式 GB/T 7714	Wang, Zhibin,Wei, Lin,He, Yi,et al. In vivo vascular imaging with adaptive optics confocal scanning fluorescence microscopy[C]. 见:Proceedings of SPIE: Twelfth International Conference on Photonics and Imaging in Biology and Medicine, PIBM 2014. 2014.

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