SNSMIL, a real-time single molecule identification and localization algorithm for super-resolution fluorescence microscopy

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	SNSMIL, a real-time single molecule identification and localization algorithm for super-resolution fluorescence microscopy
	Tang, YQ; Dai, LR; Zhang, XM; Li, JB; Hendriks, JN; Fan, XM; Gruteser, N; Meisenberg, A; Baumann, A; Katranidis, A
刊名	SCIENTIFIC REPORTS
	2015
卷号	5 期号:0 页码:11073
通讯作者	Li, JB (reprint author), Natl Ctr Nanosci & Technol China, 11 Beiyitiao Zhongguancun, Beijing 100190, Peoples R China.
英文摘要	Single molecule localization based super-resolution fluorescence microscopy offers significantly higher spatial resolution than predicted by Abbe's resolution limit for far field optical microscopy. Such super-resolution images are reconstructed from wide-field or total internal reflection single molecule fluorescence recordings. Discrimination between emission of single fluorescent molecules and background noise fluctuations remains a great challenge in current data analysis. Here we present a real-time, and robust single molecule identification and localization algorithm, SNSMIL (Shot Noise based Single Molecule Identification and Localization). This algorithm is based on the intrinsic nature of noise, i.e., its Poisson or shot noise characteristics and a new identification criterion, Q(SNSMIL), is defined. SNSMIL improves the identification accuracy of single fluorescent molecules in experimental or simulated datasets with high and inhomogeneous background. The implementation of SNSMIL relies on a graphics processing unit (GPU), making real-time analysis feasible as shown for real experimental and simulated datasets.
学科主题	Science & Technology - Other Topics
类目[WOS]	Multidisciplinary Sciences
关键词[WOS]	RESOLUTION LIMIT ; IMAGE-ANALYSIS ; NANOSCOPY ; DEPLETION ; SOFTWARE ; TRACKING ; REVEALS ; SYSTEMS ; ACTIN ; STORM
收录类别	SCI
语种	英语
内容类型	期刊论文
源URL	[http://ir.itp.ac.cn/handle/311006/20957]
专题	理论物理研究所_理论物理所1978-2010年知识产出
推荐引用方式 GB/T 7714	Tang, YQ,Dai, LR,Zhang, XM,et al. SNSMIL, a real-time single molecule identification and localization algorithm for super-resolution fluorescence microscopy[J]. SCIENTIFIC REPORTS,2015,5(0):11073.
APA	Tang, YQ.,Dai, LR.,Zhang, XM.,Li, JB.,Hendriks, JN.,...&Gensch, T.(2015).SNSMIL, a real-time single molecule identification and localization algorithm for super-resolution fluorescence microscopy.SCIENTIFIC REPORTS,5(0),11073.
MLA	Tang, YQ,et al."SNSMIL, a real-time single molecule identification and localization algorithm for super-resolution fluorescence microscopy".SCIENTIFIC REPORTS 5.0(2015):11073.