Hybridization chain reaction amplification for highly sensitive fluorescence detection of DNA with dextran coated microarrays

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	Hybridization chain reaction amplification for highly sensitive fluorescence detection of DNA with dextran coated microarrays
	Chao, J; Li, ZH; Li, J; Peng, HZ; Su, S; Li, Q; Zhu, CF; Zuo, XL; Song, SP; Wang, LH
刊名	BIOSENSORS & BIOELECTRONICS
	2016
卷号	81 期号:-页码:92—96
关键词	SURFACE-PLASMON RESONANCE CANCER-PATIENTS ON-CHIP MICRORNA CELLS IMMOBILIZATION NANOPARTICLES BIOSENSORS UNIVERSAL PLATFORM
ISSN号	0956-5663
通讯作者	Wang, LH (reprint author), Chinese Acad Sci, Shanghai Inst Appl Phys, Shanghai Synchrotron Radiat Facil, Div Phys Biol, 2019 Jialuo Rd, Shanghai 201800, Peoples R China. ; Wang, LH (reprint author), Chinese Acad Sci, Shanghai Inst Appl Phys, Shanghai Synchrotron Radiat Facil, Bioimaging Ctr, 2019 Jialuo Rd, Shanghai 201800, Peoples R China.
英文摘要	Microarrays of biomolecules hold great promise in the fields of genomics, proteomics, and clinical assays on account of their remarkably parallel and high-throughput assay capability. However, the fluorescence detection used in most conventional DNA microarrays is still limited by sensitivity. In this study, we have demonstrated a novel universal and highly sensitive platform for fluorescent detection of sequence specific DNA at the femtomolar level by combining dextran-coated microarrays with hybridization chain reaction (HCR) signal amplification. Three-dimensional dextran matrix was covalently coated on glass surface as the scaffold to immobilize DNA recognition probes to increase the surface binding capacity and accessibility. DNA nanowire tentacles were formed on the matrix surface for efficient signal amplification by capturing multiple fluorescent molecules in a highly ordered way. By quantifying microscopic fluorescent signals, the synergetic effects of dextran and HCR greatly improved sensitivity of DNA microarrays, with a detection limit of 10 fM (1 x 10(5) molecules). This detection assay could recognize one-base mismatch with fluorescence signals dropped down to 20%. This cost-effective microarray platform also worked well with samples in serum and thus shows great potential for clinical diagnosis. (C) 2016 Elsevier B.V. All rights reserved.
收录类别	SCI
语种	英语
WOS记录号	WOS:000374811800014
内容类型	期刊论文
源URL	[http://ir.sinap.ac.cn/handle/331007/25747]
专题	上海应用物理研究所_中科院上海应用物理研究所2011-2017年
推荐引用方式 GB/T 7714	Chao, J,Li, ZH,Li, J,et al. Hybridization chain reaction amplification for highly sensitive fluorescence detection of DNA with dextran coated microarrays[J]. BIOSENSORS & BIOELECTRONICS,2016,81(-):92—96.
APA	Chao, J.,Li, ZH.,Li, J.,Peng, HZ.,Su, S.,...&Wang, LH.(2016).Hybridization chain reaction amplification for highly sensitive fluorescence detection of DNA with dextran coated microarrays.BIOSENSORS & BIOELECTRONICS,81(-),92—96.
MLA	Chao, J,et al."Hybridization chain reaction amplification for highly sensitive fluorescence detection of DNA with dextran coated microarrays".BIOSENSORS & BIOELECTRONICS 81.-(2016):92—96.