An electrochemical sensor based on cellulose nanocrystal for the enantioselective discrimination of chiral amino acids | |
Bi, Qing1; Dong SQ(董树清)2; Sun YM(孙亚明)2,3; Lu XQ(卢小泉)1; Zhao L(赵亮)2; Zhao L(赵亮); Zhao L(赵亮) | |
刊名 | Analytical Biochemistry |
2016 | |
卷号 | 508期号:1页码:50-57 |
关键词 | Cellulose nanocrystals Electrochemical sensor Chiral amino acids Enantioselective discrimination |
ISSN号 | 0003-2697 |
通讯作者 | 赵亮 ; 卢小泉 |
英文摘要 | A novel electrochemical sensor based on 2,2,6,6-tetramethylpiperidine-1-oxyl (TEMPO)-oxidized cellulose nanocrystals (TOCNCs) and L-cystines (L-Cys) modified Au electrode (TOCNC/L-Cys/Au) has been fabricated for detection and discrimination of the enantiomers of phenylalanine (Phe), leucine (Leu), and valine (Val). The three amino acids are in connection with metabolism diseases. The TOCNC/L-Cys/Au electrode exhibited obvious peak current difference for the amino acid enantiomers by cyclic voltammetry (CV) and differential pulse voltammetry (DPV). The TOCNCs on the electrode surface expressed different interactions with D- and L-amino acids, so the electrochemical recognitions of the three amino acid enantiomers were achieved. TOCNCs were characterized by Fourier transform infrared (FT-IR) and scanning electron microscopy (SEM). The modified electrodes were characterized by SEM and electrochemical techniques. According to DPV, peak currents of the two enantiomers decreased linearly with their concentrations. Furthermore, satisfactory results were obtained when this electrode was applied to measure the D- and L-Phe mixture. The experimental results show that TOCNCs are suitable material for chiral sensor. The contrast of serum sample of healthy people and patients with type 2 diabetes also was proposed, and significant difference was exhibited on the modified electrode. This work is significant for the screening, diagnosis, and treatment of multiple metabolic diseases. |
学科主题 | 分析化学与药物化学 |
收录类别 | SCI |
资助信息 | the National Natural Science Foundation of China (21405161;21405162;21575115;21327005;21175108);the Program for Chang Jiang Scholars and Innovative Research Team, Ministry of Education, China (IRT1283) |
语种 | 英语 |
WOS记录号 | WOS:000380623400010 |
内容类型 | 期刊论文 |
源URL | [http://210.77.64.217/handle/362003/20220] |
专题 | 兰州化学物理研究所_中科院西北特色植物资源化学重点实验室/甘肃省天然药物重点实验室 |
通讯作者 | Zhao L(赵亮); Zhao L(赵亮) |
作者单位 | 1.Northwest Normal Univ, Coll Chem & Chem Engn, Key Lab Bioelectrochem & Environm Anal Gansu Prov, Lanzhou 730070, Peoples R China 2.Chinese Acad Sci, Lanzhou Inst Chem Phys, Lanzhou 730070, Peoples R China 3.Chinese Acad Sci, Grad Univ, Beijing 100080, Peoples R China |
推荐引用方式 GB/T 7714 | Bi, Qing,Dong SQ,Sun YM,et al. An electrochemical sensor based on cellulose nanocrystal for the enantioselective discrimination of chiral amino acids[J]. Analytical Biochemistry,2016,508(1):50-57. |
APA | Bi, Qing.,Dong SQ.,Sun YM.,Lu XQ.,Zhao L.,...&赵亮.(2016).An electrochemical sensor based on cellulose nanocrystal for the enantioselective discrimination of chiral amino acids.Analytical Biochemistry,508(1),50-57. |
MLA | Bi, Qing,et al."An electrochemical sensor based on cellulose nanocrystal for the enantioselective discrimination of chiral amino acids".Analytical Biochemistry 508.1(2016):50-57. |
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