Functional Analysis of the Isopentenyl Diphosphate Isomerase of Salvia miltiorrhiza via Color Complementation and RNA Interference

	Functional Analysis of the Isopentenyl Diphosphate Isomerase of Salvia miltiorrhiza via Color Complementation and RNA Interference
	Zhang, Xianan 1; Guan, Hongyu 1; Dai, Zhubo 2; Guo, Juan 3; Shen, Ye 3; Cui, Guanghong 3; Gao, Wei 1; Huang, Luqi 3
刊名	MOLECULES
	2015-11-01
卷号	20 期号:11 页码:20206-20218
关键词	isopentenyl diphosphate isomerase color complementation RNA interference Salvia miltiorrhiza functional identification
英文摘要	Isopentenyl diphosphate isomerase (IPI) catalyzes the isomerization between the common terpene precursor substances isopentenyl diphosphate (IPP) and dimethylallyl diphosphate (DMAPP) during the terpenoid biosynthesis process. In this study, tissue expression analysis revealed that the expression level of the Salvia miltiorrhiza IPI1 gene (SmIPI1) was higher in the leaves than in the roots and stems. Furthermore, color complementation and RNA interference methods were used to verify the function of the SmIPI1 gene from two aspects. A recombinant SmIPI1 plasmid was successfully constructed and transferred into engineered E. coli for validating the function of SmIPI1 through the color difference in comparison to the control group; the observed color difference indicated that SmIPI1 served in promoting the accumulation of lycopene. Transformant hairy root lines with RNA interference of SmIPI1 were successfully constructed mediated by Agrobacterium rhizogenes ACCC 10060. RNA interference hairy roots had a severe phenotype characterized by withering, deformity or even death. The mRNA expression level of SmIPI1 in the RSi3 root line was only 8.4% of that of the wild type. Furthermore the tanshinone content was too low to be detected in the RNA interference lines. These results suggest that SmIPI1 plays a critical role in terpenoid metabolic pathways. Addition of an exogenous SmIPI1 gene promoted metabolic flow toward the biosynthesis of carotenoids in E. coli, and SmIPI1 interference in S. miltiorrhiza hairy roots may cause interruption of the 2-C-methyl-D-erythritol-4-phosphate metabolic pathway.
WOS标题词	Science & Technology ; Physical Sciences
类目[WOS]	Chemistry, Organic
研究领域[WOS]	Chemistry
关键词[WOS]	ISOPRENOID BIOSYNTHESIS ; ESCHERICHIA-COLI ; DIMETHYLALLYL DIPHOSPHATE ; SACCHAROMYCES-CEREVISIAE ; MEVALONATE PATHWAY ; LYCOPENE CYCLASE ; GENE ; ARABIDOPSIS ; ACCUMULATION ; EXPRESSION
收录类别	SCI
语种	英语
WOS记录号	WOS:000365924800041
内容类型	期刊论文
源URL	[http://124.16.173.210/handle/834782/1553]
专题	天津工业生物技术研究所_微生物代谢工程张学礼_期刊论文
作者单位	1.Capital Med Univ, Sch Tradit Chinese Med, Beijing 100069, Peoples R China 2.Chinese Acad Sci, Tianjin Inst Ind Biotechnol, Key Lab Syst Microbial Biotechnol, Tianjin 300308, Peoples R China 3.Chinese Acad Chinese Med Sci, Inst Chinese Mat Med, Beijing 100700, Peoples R China
推荐引用方式 GB/T 7714	Zhang, Xianan,Guan, Hongyu,Dai, Zhubo,et al. Functional Analysis of the Isopentenyl Diphosphate Isomerase of Salvia miltiorrhiza via Color Complementation and RNA Interference[J]. MOLECULES,2015,20(11):20206-20218.
APA	Zhang, Xianan.,Guan, Hongyu.,Dai, Zhubo.,Guo, Juan.,Shen, Ye.,...&Huang, Luqi.(2015).Functional Analysis of the Isopentenyl Diphosphate Isomerase of Salvia miltiorrhiza via Color Complementation and RNA Interference.MOLECULES,20(11),20206-20218.
MLA	Zhang, Xianan,et al."Functional Analysis of the Isopentenyl Diphosphate Isomerase of Salvia miltiorrhiza via Color Complementation and RNA Interference".MOLECULES 20.11(2015):20206-20218.