Engineering central metabolic modules of Escherichia coli for improving beta-carotene production

	Engineering central metabolic modules of Escherichia coli for improving beta-carotene production
	Zhao, Jing 1,2,3; Li, Qingyan 1,2; Sun, Tao 1,2,3; Zhu, Xinna 1,2; Xu, Hongtao 1,2; Tang, Jinlei 1,2,3; Zhang, Xueli 1,2; Ma, Yanhe 1
刊名	METABOLIC ENGINEERING
	2013-05-01
卷号	17 页码:42-50
关键词	beta-Carotene Terpenoids Cofactor NADPH Escherichia coli
英文摘要	ATP and NADPH are two important cofactors for production of terpenoids compounds. Here we have constructed and optimized beta-carotene synthetic pathway in Escherichia coli, followed by engineering central metabolic modules to increase ATP and NADPH supplies for improving beta-carotene production. The whole beta-carotene synthetic pathway was divided into five modules. Engineering MEP module resulted in 3.5-fold increase of beta-carotene yield, while engineering beta-carotene synthesis module resulted in another 3.4-fold increase. The best beta-carotene yield increased 21%, 17% and 39% after modulating single gene of ATP synthesis, pentose phosphate and TCA modules, respectively. Combined engineering of TCA and PPP modules had a synergistic effect on improving beta-carotene yield, leading to 64% increase of beta-carotene yield over a high producing parental strain. Fed-batch fermentation of the best strain CAR005 was performed, which produced 2.1 g/L beta-carotene with a yield of 60 mg/g DCW. (C) 2013 Elsevier Inc. All rights reserved.
WOS标题词	Science & Technology ; Life Sciences & Biomedicine
类目[WOS]	Biotechnology & Applied Microbiology
研究领域[WOS]	Biotechnology & Applied Microbiology
关键词[WOS]	LYCOPENE PRODUCTION ; ISOPRENOID PATHWAY ; CHROMOSOMAL GENES ; EXPRESSION ; MICROORGANISMS ; BIOSYNTHESIS
收录类别	SCI
语种	英语
WOS记录号	WOS:000318583700005
内容类型	期刊论文
源URL	[http://124.16.173.210/handle/834782/1239]
专题	天津工业生物技术研究所_微生物代谢工程张学礼_期刊论文
作者单位	1.Chinese Acad Sci, Tianjin Inst Ind Biotechnol, Tianjin 300308, Peoples R China 2.Chinese Acad Sci, Key Lab Syst Microbial Biotechnol, Tianjin 300308, Peoples R China 3.Tianjin Univ Sci & Technol, Coll Biotechnol, Tianjin 300308, Peoples R China
推荐引用方式 GB/T 7714	Zhao, Jing,Li, Qingyan,Sun, Tao,et al. Engineering central metabolic modules of Escherichia coli for improving beta-carotene production[J]. METABOLIC ENGINEERING,2013,17:42-50.
APA	Zhao, Jing.,Li, Qingyan.,Sun, Tao.,Zhu, Xinna.,Xu, Hongtao.,...&Ma, Yanhe.(2013).Engineering central metabolic modules of Escherichia coli for improving beta-carotene production.METABOLIC ENGINEERING,17,42-50.
MLA	Zhao, Jing,et al."Engineering central metabolic modules of Escherichia coli for improving beta-carotene production".METABOLIC ENGINEERING 17(2013):42-50.