NADP(+)-Preferring D-Lactate Dehydrogenase from Sporolactobacillus inulinus

	NADP(+)-Preferring D-Lactate Dehydrogenase from Sporolactobacillus inulinus
	Zhu, Lingfeng 1,6; Xu, Xiaoling 4; Wang, Limin 1; Dong, Hui 2,3; Yu, Bo 1; Ma, Yanhe 5
刊名	APPLIED AND ENVIRONMENTAL MICROBIOLOGY
	2015-09-01
卷号	81 期号:18 页码:6294-6301
英文摘要	Hydroxy acid dehydrogenases, including L-and D-lactate dehydrogenases (L-LDH and D-LDH), are responsible for the stereospecific conversion of 2-keto acids to 2-hydroxyacids and extensively used in a wide range of biotechnological applications. A common feature of LDHs is their high specificity for NAD(+) as a cofactor. An LDH that could effectively use NADPH as a coenzyme could be an alternative enzymatic system for regeneration of the oxidized, phosphorylated cofactor. In this study, a D-lactate dehydrogenase from a Sporolactobacillus inulinus strain was found to use both NADH and NADPH with high efficiencies and with a preference for NADPH as its coenzyme, which is different from the coenzyme utilization of all previously reported LDHs. The biochemical properties of the D-LDH enzyme were determined by X-ray crystal structural characterization and in vivo and in vitro enzymatic activity analyses. The residue Asn(174) was demonstrated to be critical for NADPH utilization. Characterization of the biochemical properties of this enzyme will contribute to understanding of the catalytic mechanism and provide referential information for shifting the coenzyme utilization specificity of 2-hydroxyacid dehydrogenases.
WOS标题词	Science & Technology ; Life Sciences & Biomedicine
类目[WOS]	Biotechnology & Applied Microbiology ; Microbiology
研究领域[WOS]	Biotechnology & Applied Microbiology ; Microbiology
关键词[WOS]	LACTIC-ACID ; 2-HYDROXYACID DEHYDROGENASE ; EFFICIENT PRODUCTION ; ESCHERICHIA-COLI ; OPTICAL PURITY ; SP STRAIN ; BULGARICUS ; SPECIFICITY ; TOLERANCE ; SEQUENCE
收录类别	SCI
语种	英语
WOS记录号	WOS:000362676400020
内容类型	期刊论文
源URL	[http://124.16.173.210/handle/834782/1564]
专题	天津工业生物技术研究所_酶工程实验室马延和 _期刊论文
作者单位	1.Chinese Acad Sci, CAS Key Lab Microbial Physiol & Metab Engn, Inst Microbiol, Beijing, Peoples R China 2.Chinese Acad Med Sci, Key Lab Tianjin Radiat & Mol Nucl Med, Inst Radiat Med, Tianjin, Peoples R China 3.Peking Union Med Coll, Tianjin, Peoples R China 4.Hangzhou Normal Univ, Inst Ageing Res, Sch Med, Hangzhou, Zhejiang, Peoples R China 5.Chinese Acad Sci, Tianjin Inst Ind Biotechnol, Tianjin, Peoples R China 6.Univ Chinese Acad Sci, Beijing, Peoples R China
推荐引用方式 GB/T 7714	Zhu, Lingfeng,Xu, Xiaoling,Wang, Limin,et al. NADP(+)-Preferring D-Lactate Dehydrogenase from Sporolactobacillus inulinus[J]. APPLIED AND ENVIRONMENTAL MICROBIOLOGY,2015,81(18):6294-6301.
APA	Zhu, Lingfeng,Xu, Xiaoling,Wang, Limin,Dong, Hui,Yu, Bo,&Ma, Yanhe.(2015).NADP(+)-Preferring D-Lactate Dehydrogenase from Sporolactobacillus inulinus.APPLIED AND ENVIRONMENTAL MICROBIOLOGY,81(18),6294-6301.
MLA	Zhu, Lingfeng,et al."NADP(+)-Preferring D-Lactate Dehydrogenase from Sporolactobacillus inulinus".APPLIED AND ENVIRONMENTAL MICROBIOLOGY 81.18(2015):6294-6301.