Mesoporous bioactive glass nanolayer-functionalized 3D-printed scaffolds for accelerating osteogenesis and angiogenesis

CORC > 上海硅酸盐研究所 > 中国科学院上海硅酸盐研究所 > 生物材料与组织工程研究中心 > 期刊论文

	Mesoporous bioactive glass nanolayer-functionalized 3D-printed scaffolds for accelerating osteogenesis and angiogenesis
	Zhang, Yali 1,3; Xia, Lunguo 2; Zhai, Dong 1; Shi, Mengchao 1; Luo, Yongxiang 1; Feng, Chun 1; Fang, Bing 2; Yin, Jingbo 3; Chang, Jiang 1; Wu, Chengtie 1
刊名	NANOSCALE
	2015
卷号	7 期号:45 页码:19207-19221
英文摘要	The hierarchical microstructure, surface and interface of biomaterials are important factors influencing their bioactivity. Porous bioceramic scaffolds have been widely used for bone tissue engineering by optimizing their chemical composition and large-pore structure. However, the surface and interface of struts in bioceramic scaffolds are often ignored. The aim of this study is to incorporate hierarchical pores and bioactive components into the bioceramic scaffolds by constructing nanopores and bioactive elements on the struts of scaffolds and further improve their bone-forming activity. Mesoporous bioactive glass (MBG) modified beta-tricalcium phosphate (MBG-beta-TCP) scaffolds with a hierarchical pore structure and a functional strut surface (similar to 100 nm of MBG nanolayer) were successfully prepared via 3D printing and spin coating. The compressive strength and apatite-mineralization ability of MBG-beta-TCP scaffolds were significantly enhanced as compared to beta-TCP scaffolds without the MBG nanolayer. The attachment, viability, alkaline phosphatase (ALP) activity, osteogenic gene expression (Runx2, BMP2, OPN and Col I) and protein expression (OPN, Col I, VEGF, HIF-1 alpha) of rabbit bone marrow stromal cells (rBMSCs) as well as the attachment, viability and angiogenic gene expression (VEGF and HIF-1 alpha) of human umbilical vein endothelial cells (HUVECs) in MBG-beta-TCP scaffolds were significantly upregulated compared with conventional bioactive glass (BG)-modified beta-TCP (BG-beta-TCP) and pure beta-TCP scaffolds. Furthermore, MBG-beta-TCP scaffolds significantly enhanced the formation of new bone in vivo as compared to BG-beta-TCP and beta-TCP scaffolds. The results suggest that application of the MBG nanolayer to modify 3D-printed bioceramic scaffolds offers a new strategy to construct hierarchically porous scaffolds with significantly improved physicochemical and biological properties, such as mechanical properties, osteogenesis, angiogenesis and protein expression for bone tissue engineering applications, in which the incorporation of nanostructures and bioactive components into the scaffold struts synergistically play a key role in the improved bone formation.
WOS标题词	Science & Technology ; Physical Sciences ; Technology
类目[WOS]	Chemistry, Multidisciplinary ; Nanoscience & Nanotechnology ; Materials Science, Multidisciplinary ; Physics, Applied
研究领域[WOS]	Chemistry ; Science & Technology - Other Topics ; Materials Science ; Physics
关键词[WOS]	MESENCHYMAL STEM-CELLS ; CALCIUM-PHOSPHATE ; IONIC PRODUCTS ; IN-VITRO ; PROTEIN ADSORPTION ; BONE-FORMATION ; SURFACE ; DIFFERENTIATION ; HYDROXYAPATITE ; STIMULATION
收录类别	SCI
语种	英语
WOS记录号	WOS:000364852500048
内容类型	期刊论文
源URL	[http://ir.sic.ac.cn/handle/331005/8639]
专题	上海硅酸盐研究所_生物材料与组织工程研究中心_期刊论文
作者单位	1.Chinese Acad Sci, Shanghai Inst Ceram, State Key Lab High Performance Ceram & Superfine, Shanghai 200050, Peoples R China 2.Shanghai Jiao Tong Univ, Peoples Hosp Affiliated 9, Sch Med, Ctr Craniofacial Orthodont,Dept Oral & Craniomax, Shanghai 200011, Peoples R China 3.Shanghai Univ, Dept Polymer Mat, Shanghai 200444, Peoples R China
推荐引用方式 GB/T 7714	Zhang, Yali,Xia, Lunguo,Zhai, Dong,et al. Mesoporous bioactive glass nanolayer-functionalized 3D-printed scaffolds for accelerating osteogenesis and angiogenesis[J]. NANOSCALE,2015,7(45):19207-19221.
APA	Zhang, Yali.,Xia, Lunguo.,Zhai, Dong.,Shi, Mengchao.,Luo, Yongxiang.,...&Wu, Chengtie.(2015).Mesoporous bioactive glass nanolayer-functionalized 3D-printed scaffolds for accelerating osteogenesis and angiogenesis.NANOSCALE,7(45),19207-19221.
MLA	Zhang, Yali,et al."Mesoporous bioactive glass nanolayer-functionalized 3D-printed scaffolds for accelerating osteogenesis and angiogenesis".NANOSCALE 7.45(2015):19207-19221.