Irregular xylem 7 (IRX7) is required for anchoring seed coat mucilage in Arabidopsis

CORC > 青岛生物能源与过程研究所 > 中国科学院青岛生物能源与过程研究所 > 植物代谢工程团队

	Irregular xylem 7 (IRX7) is required for anchoring seed coat mucilage in Arabidopsis
	Hu, Ruibo 1; Li, Junling 1; Yang, Xuanwen 1; Zhao, Xun 1; Wang, Xiaoyu 1; Tang, Qi 1; He, Guo 1; Zhou, Gongke 1; Kong, Yingzhen 2
刊名	PLANT MOLECULAR BIOLOGY
	2016-09-01
卷号	92 期号:1-2 页码:25-38
关键词	Arabidopsis Irregular Xylem 7 Mucilage Xylan Crystalline cellulose
英文摘要	Large quantities of mucilage are synthesized in seed coat epidermis cells during seed coat differentiation. This process is an ideal model system for the study of plant cell wall biosynthesis and modifications. In this study, we show that mutation in Irregular Xylem 7 (IRX7) results in a defect in mucilage adherence due to reduced xylan biosynthesis. IRX7 was expressed in the seeds from 4 days post-anthesis (DPA) to 13 DPA, with the peak of expression at 13 DPA. The seed coat epidermis cells of irx7 displayed no aberrant morphology during differentiation, and these cells synthesized and deposited the same amount of mucilage as did wild type (WT) cells. However, the distribution of the water-soluble vs. adherent mucilage layers was significantly altered in irx7 compared to the WT. Both the amount of xylose and the extent of glycosyl linkages of xylan was dramatically decreased in irx7 water-soluble and adherent mucilage compared to the WT. The polymeric structure of water-soluble mucilage was altered in irx7, with a total loss of the higher molecular weight polymer components present in the WT. Correspondingly, whole-seed immunolabeling assays and dot-immunoassays of extracted mucilage indicated dramatic changes in rhamnogalacturonan I (RG I) and xylan epitopes in irx7 mucilage. Furthermore, the crystalline cellulose content was significantly reduced in irx7 mucilage. Taken together, these results indicate that xylan synthesized by IRX7 plays an essential role in maintaining the adhesive property of seed coat mucilage, and its structural role is potentially implemented through its interaction with cellulose.
WOS标题词	Science & Technology ; Life Sciences & Biomedicine
类目[WOS]	Biochemistry & Molecular Biology ; Plant Sciences
研究领域[WOS]	Biochemistry & Molecular Biology ; Plant Sciences
关键词[WOS]	PLANT-CELL WALL ; REDUCING END-GROUPS ; GLUCURONOXYLAN BIOSYNTHESIS ; SECRETORY-CELLS ; GLYCOSYL TRANSFERASES ; MONOCLONAL-ANTIBODIES ; CELLULOSE STRUCTURE ; ADHERENT MUCILAGE ; XYLAN SYNTHESIS ; IN-SITU
收录类别	SCI
语种	英语
WOS记录号	WOS:000382336500002
内容类型	期刊论文
源URL	[http://ir.qibebt.ac.cn/handle/337004/8488]
专题	青岛生物能源与过程研究所_植物代谢工程团队
作者单位	1.Chinese Acad Sci, Qingdao Inst Bioenergy & Bioproc Technol, Qingdao Engn Res Ctr Biomass Resources & Environm, Qingdao 266101, Peoples R China 2.Chinese Acad Agr Sci, Tobacco Res Inst, Qingdao 266101, Qingdao, Peoples R China
推荐引用方式 GB/T 7714	Hu, Ruibo,Li, Junling,Yang, Xuanwen,et al. Irregular xylem 7 (IRX7) is required for anchoring seed coat mucilage in Arabidopsis[J]. PLANT MOLECULAR BIOLOGY,2016,92(1-2):25-38.
APA	Hu, Ruibo.,Li, Junling.,Yang, Xuanwen.,Zhao, Xun.,Wang, Xiaoyu.,...&Kong, Yingzhen.(2016).Irregular xylem 7 (IRX7) is required for anchoring seed coat mucilage in Arabidopsis.PLANT MOLECULAR BIOLOGY,92(1-2),25-38.
MLA	Hu, Ruibo,et al."Irregular xylem 7 (IRX7) is required for anchoring seed coat mucilage in Arabidopsis".PLANT MOLECULAR BIOLOGY 92.1-2(2016):25-38.