Synergistic effect between the recombinant exo-inulinase and endo-inulinase on inulin hydrolysis

CORC > 青岛生物能源与过程研究所 > 中国科学院青岛生物能源与过程研究所 > 代谢物组学团队

	Synergistic effect between the recombinant exo-inulinase and endo-inulinase on inulin hydrolysis
	Liu, Yuan 1; Zhou, Shun-Hua 1; Cheng, Yu-Rong 2; Chi, Zhe 1; Chi, Zhen-Ming 1; Liu, Guang-Lei 1
刊名	JOURNAL OF MOLECULAR CATALYSIS B-ENZYMATIC
	2016-06-01
卷号	128 页码:27-38
关键词	Synergistic effect Exo-inulinase Endo-inulinase Inulin Inulo-oligosaccharides
英文摘要	An exo-inulinase gene from Kluyveromyces marxianus and an endo-inulinase gene from Aspergillus niger were cloned and expressed in Yarrowia lipolytica, respectively. Then, the purified recombinant exo-inulinase (rEXINU) and endo-inulinase (rENINU), either individual or combination with another, were used for hydrolysis of inulin and inulin in tuber meal of Jerusalem artichoke. After the analysis of the dynamic hydrolysis process, it was found that the rEXINU and the rENINU acted synergistically with each other, and the maximum degree of synergistic effect on the level of fructose formation (DSEFru) could be obtained with a molar ratio of the rEXINU to the rENINU at 1:1. Furthermore, the investigation of the hydrolysis product in the synergistic hydrolysis process showed that a wide range of oligosaccharides were first generated and then converted to fructose. Based on the enzyme kinetics, substrate specificity and product inhibition of the rEXINU and the rENINU, a synergism mechanism of the them for the inulin hydrolysis was proposed. During the process of the synergistic action, the rENINU preferentially degraded the inulin with high DP, but was inhibited by the produced oligosaccharides. Then the released oligosaccharides which were the optimum substrates of the rEXIUN were rapidly hydrolyzed by the enzyme to produce fructose and glucose. Meanwhile, this process relieved the inhibition of the produced oligosaccharides to the rENINU. Besides, in the synergistic hydrolysis of the inulin in tuber meal of Jerusalem artichoke, the depolymerization of the rENINU could result in the increase of the subtrate solubility to improve the hydrolysis efficiency. Therefore, this kind of the synergistic effect between the exo-inulinase and the endo-inulinase may provide a potential driving force in the industrial application of inulin and inulin-containing materials. (C) 2016 Elsevier B.V. All rights reserved.
WOS标题词	Science & Technology ; Life Sciences & Biomedicine ; Physical Sciences
类目[WOS]	Biochemistry & Molecular Biology ; Chemistry, Physical
研究领域[WOS]	Biochemistry & Molecular Biology ; Chemistry
关键词[WOS]	HETEROLOGOUS PROTEIN EXPRESSION ; SACCHAROMYCES-CEREVISIAE ; KLUYVEROMYCES-MARXIANUS ; ENHANCED EXPRESSION ; YARROWIA-LIPOLYTICA ; ASPERGILLUS-NIGER ; ALPHA-AMYLASE ; HYDROLASE ; GENE ; GUILLIERMONDII
收录类别	SCI
语种	英语
WOS记录号	WOS:000375008100004
内容类型	期刊论文
源URL	[http://ir.qibebt.ac.cn/handle/337004/8268]
专题	青岛生物能源与过程研究所_代谢物组学团队
作者单位	1.Ocean Univ China, Yushan Rd 5, Qingdao 266003, Shandong, Peoples R China 2.Chinese Acad Sci, Qingdao Inst Bioenergy & Bioproc Technol, Songling Rd 189, Qingdao 266101, Shandong, Peoples R China
推荐引用方式 GB/T 7714	Liu, Yuan,Zhou, Shun-Hua,Cheng, Yu-Rong,et al. Synergistic effect between the recombinant exo-inulinase and endo-inulinase on inulin hydrolysis[J]. JOURNAL OF MOLECULAR CATALYSIS B-ENZYMATIC,2016,128:27-38.
APA	Liu, Yuan,Zhou, Shun-Hua,Cheng, Yu-Rong,Chi, Zhe,Chi, Zhen-Ming,&Liu, Guang-Lei.(2016).Synergistic effect between the recombinant exo-inulinase and endo-inulinase on inulin hydrolysis.JOURNAL OF MOLECULAR CATALYSIS B-ENZYMATIC,128,27-38.
MLA	Liu, Yuan,et al."Synergistic effect between the recombinant exo-inulinase and endo-inulinase on inulin hydrolysis".JOURNAL OF MOLECULAR CATALYSIS B-ENZYMATIC 128(2016):27-38.