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	In vivo imaging of mice auricle vessels using adaptive optical confocal fluorescence microscope
	He, Yi1,2; Wang, Zhibin1,2,4; Wang, Yuanyuan3,4; Wei, Ling1,2; Li, Xiqi1,2; Yang, Jinsheng1,2; Shi, Guohua1,2; Zhang, Yudong1,2
刊名	Chinese Optics Letters
	2015
卷号	13期号:11页码:111702
ISSN号	1671-7694
通讯作者	He, Yi (heyi_job@126.com)
中文摘要	Facilitated with stochastic parallel gradient descent (SPGD) algorithm for wavefront sensorless correcting aberrations, an adaptive optics (AO) confocal fluorescence microscopy is developed and used to record fluorescent signals in vivo. Vessels of mice auricle at 80, 100 and 120 μm depth are obtained, and image contrast and fluorescence intensity are significantly improved with AO correction. The typical 10%-90% rise-time of the metric value measured is 5.0 s for a measured close-loop bandwidth of 0.2 Hz. Therefore, the AO confocal microscopy implemented with SPGD algorithm for robust AO corrections will be a powerful tool for study of vascular dynamics in future. © 2015 Chinese Optics Letters.
英文摘要	Facilitated with stochastic parallel gradient descent (SPGD) algorithm for wavefront sensorless correcting aberrations, an adaptive optics (AO) confocal fluorescence microscopy is developed and used to record fluorescent signals in vivo. Vessels of mice auricle at 80, 100 and 120 μm depth are obtained, and image contrast and fluorescence intensity are significantly improved with AO correction. The typical 10%-90% rise-time of the metric value measured is 5.0 s for a measured close-loop bandwidth of 0.2 Hz. Therefore, the AO confocal microscopy implemented with SPGD algorithm for robust AO corrections will be a powerful tool for study of vascular dynamics in future. © 2015 Chinese Optics Letters.
学科主题	Algorithms - Bandwidth - Fluorescence - Fluorescence microscopy - Gradient methods - Mammals - Stochastic systems - Underwater acoustics
收录类别	SCI ; EI
语种	英语
WOS记录号	WOS:000364520000016
内容类型	期刊论文
源URL	[http://ir.ioe.ac.cn/handle/181551/6416]
专题	光电技术研究所_自适应光学技术研究室（八室）
作者单位	1.The Key Laboratory on Adaptive Optics, Chinese Academy of Sciences, Chengdu, China 2.The Laboratory on Adaptive Optics, Institute of Optics and Electronics, Chinese Academy of Sciences, Chengdu, China 3.School of Optometry and Ophthalmology and Eye Hospital, Wenzhou Medical College, Wenzhou, China 4.University of Chinese Academy of Sciences, Beijing, China
推荐引用方式 GB/T 7714	He, Yi,Wang, Zhibin,Wang, Yuanyuan,et al. In vivo imaging of mice auricle vessels using adaptive optical confocal fluorescence microscope[J]. Chinese Optics Letters,2015,13(11):111702.
APA	He, Yi.,Wang, Zhibin.,Wang, Yuanyuan.,Wei, Ling.,Li, Xiqi.,...&Zhang, Yudong.(2015).In vivo imaging of mice auricle vessels using adaptive optical confocal fluorescence microscope.Chinese Optics Letters,13(11),111702.
MLA	He, Yi,et al."In vivo imaging of mice auricle vessels using adaptive optical confocal fluorescence microscope".Chinese Optics Letters 13.11(2015):111702.

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