Programmed cell death in the cyanobacterium Microcystis aeruginosa induced by allelopathic effect of submerged macrophyte Myriophyllum spicatum in co-culture system

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	Programmed cell death in the cyanobacterium Microcystis aeruginosa induced by allelopathic effect of submerged macrophyte Myriophyllum spicatum in co-culture system
	He, Yan 1,2; Zhou, Qiao-Hong 1; Liu, Bi-Yun 1; Cheng, Long 1; Tian, Yun 1; Zhang, Yong-Yuan 1; Wu, Zhen-Bin 1
刊名	JOURNAL OF APPLIED PHYCOLOGY
	2016-10-01
卷号	28 期号:5 页码:2805-2814
关键词	Microcystis aeruginosa Myriophyllum spicatum Allelopathic effect ROS/NO Caspase-3-like activity Programmed cell death
ISSN号	0921-8971
英文摘要	This investigation demonstrates that programmed cell death (PCD) in a cyanobacterium, Microcystis aeruginosa, resulting from allelopathic stress induced by a submerged macrophyte, Myriophyllum spicatum, in a co-culture system. The hallmarks of PCD, caspase-3-like protease activity, DNA fragmentation, and destruction of cell ultrastructure, as well as intracellular PCD signaling radicals, reactive oxygen species (ROS), and nitric oxide (NO), were measured in M. aeruginosa cells co-cultured with M. spicatum for 7 days. The results showed a dose-response relationship between M. spicatum biomass and M. aeruginosa mortality. A caspase-3-like protease was activated and elevated from day 3. Thylakoid disintegration, cytoplasmic vacuolation, and fuzzy nuclear zone were observed by transmission electron microscopy, and distinct DNA fragmentation was detected in M. aeruginosa cells at a M. spicatum biomass of 6.0 g fresh weight (FW) L-1 during the 7 days. Allelochemicals of total phenolic compounds (TPCs) were determined in co-culture water, and the concentration increased with increasing of M. spicatum biomass and co-culture time. Compared with the level of ROS production in the control group, a significant overproduction of ROS was detected in M. aeruginosa cells in the treatment group, and this was positively correlated with TPC concentration. Furthermore, the level of intracellular NO increased with the percent mortality of M. aeruginosa. The results indicated that a PCD pathway was induced in the cyanobacterium M. aeruginosa when co-cultured with the submerged macrophyte M. spicatum.
WOS标题词	Science & Technology ; Life Sciences & Biomedicine
类目[WOS]	Biotechnology & Applied Microbiology ; Marine & Freshwater Biology
研究领域[WOS]	Biotechnology & Applied Microbiology ; Marine & Freshwater Biology
关键词[WOS]	GREEN-ALGA MICRASTERIAS ; NITRIC-OXIDE ; GROWTH-INHIBITION ; (-)-EPIGALLOCATECHIN GALLATE ; CHLAMYDOMONAS-REINHARDTII ; HYDROGEN-PEROXIDE ; SHALLOW LAKES ; STRESS ; APOPTOSIS ; MECHANISMS
收录类别	SCI
语种	英语
WOS记录号	WOS:000383569600018
内容类型	期刊论文
源URL	[http://ir.ihb.ac.cn/handle/342005/28393]
专题	水生生物研究所_鱼类生物学及渔业生物技术研究中心_学位论文
作者单位	1.Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Hubei, Peoples R China 2.Univ Chinese Acad Sci, Beijing 100049, Peoples R China
推荐引用方式 GB/T 7714	He, Yan,Zhou, Qiao-Hong,Liu, Bi-Yun,et al. Programmed cell death in the cyanobacterium Microcystis aeruginosa induced by allelopathic effect of submerged macrophyte Myriophyllum spicatum in co-culture system[J]. JOURNAL OF APPLIED PHYCOLOGY,2016,28(5):2805-2814.
APA	He, Yan.,Zhou, Qiao-Hong.,Liu, Bi-Yun.,Cheng, Long.,Tian, Yun.,...&Wu, Zhen-Bin.(2016).Programmed cell death in the cyanobacterium Microcystis aeruginosa induced by allelopathic effect of submerged macrophyte Myriophyllum spicatum in co-culture system.JOURNAL OF APPLIED PHYCOLOGY,28(5),2805-2814.
MLA	He, Yan,et al."Programmed cell death in the cyanobacterium Microcystis aeruginosa induced by allelopathic effect of submerged macrophyte Myriophyllum spicatum in co-culture system".JOURNAL OF APPLIED PHYCOLOGY 28.5(2016):2805-2814.