Development of Saccharina japonica genomic SSR markers using next-generation sequencing

CORC > 海洋研究所 > 中国科学院海洋研究所 > 实验海洋生物学重点实验室

	Development of Saccharina japonica genomic SSR markers using next-generation sequencing
	Li, Qiuying1,2 ; Zhang, Jie1,2 ; Yao, Jianting 1; Wang, Xiuliang 1; Duan, Delin 1
刊名	JOURNAL OF APPLIED PHYCOLOGY
	2016-04-01
卷号	28 期号:2 页码:1387-1390
关键词	Microsatellite Saccharina japonica Simple sequence repeats Phaeophyta
英文摘要	Illumina HiSeq 2000 next-generation sequencing (NGS) was used on Saccharina japonica specimens to develop SSRs from high-quality genomic sequences. There were 181,595 SSR loci identified using the MISA program. SSR repeat types are plentiful in genomic sequences of S. japonica, with the most abundant types being mononucleotides (54 %) and trinucleotides (26 %). Six hundred primer pairs were selected and synthesized from 19,658 primer pairs, showing 166 polymorphic SSR loci (28 %) in three selected S. japonica individuals. Sixty polymorphic SSRs were further analyzed in 24 S. japonica individuals, showing two to seven alleles for each locus (average 3.7 alleles), with observed heterozygosities of 0 similar to 0.625 and expected heterozygosities of 0.082 similar to 0.745. Polymorphism information content (PIC) values ranged from 0.08 to 0.69. Sixteen loci were classified as informative markers (PIC > 0.5). Polymorphic SSR markers developed in this study should be useful for examining genetic diversity, assessment of population structure, QTL mapping, and marker-assisted selection in S. japonica.
收录类别	SCI
语种	英语
WOS记录号	WOS:000372256900067
内容类型	期刊论文
源URL	[http://ir.qdio.ac.cn/handle/337002/126208]
专题	海洋研究所_实验海洋生物学重点实验室
作者单位	1.Chinese Acad Sci, Inst Oceanol, Key Lab Expt Marine Biol, Qingdao 266071, Peoples R China 2.Univ Chinese Acad Sci, Beijing 100049, Peoples R China
推荐引用方式 GB/T 7714	Li, Qiuying,Zhang, Jie,Yao, Jianting,et al. Development of Saccharina japonica genomic SSR markers using next-generation sequencing[J]. JOURNAL OF APPLIED PHYCOLOGY,2016,28(2):1387-1390.
APA	Li, Qiuying,Zhang, Jie,Yao, Jianting,Wang, Xiuliang,&Duan, Delin.(2016).Development of Saccharina japonica genomic SSR markers using next-generation sequencing.JOURNAL OF APPLIED PHYCOLOGY,28(2),1387-1390.
MLA	Li, Qiuying,et al."Development of Saccharina japonica genomic SSR markers using next-generation sequencing".JOURNAL OF APPLIED PHYCOLOGY 28.2(2016):1387-1390.