Lys63-linked ubiquitin chain adopts multiple conformational states for specific target recognition

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	Lys63-linked ubiquitin chain adopts multiple conformational states for specific target recognition
	Liu, Zhu 1,2,3; Gong, Zhou 1; Jiang, Wen-Xue 1; Yang, Ju 1; Zhu, Wen-Kai 1; Guo, Da-Chuan 1; Zhang, Wei-Ping 2,3; Liu, Mai-Li 1; Tang, Chun 1
刊名	ELIFE
	2015-06-19
卷号	4
英文摘要	A polyubiquitin comprises multiple covalently linked ubiquitins and recognizes myriad targets. Free or bound to ligands, polyubiquitins are found in different arrangements of ubiquitin subunits. To understand the structural basis for polyubiquitin quaternary plasticity and to explore the target recognition mechanism, we characterize the conformational space of Lys63-linked diubiquitin (K63-Ub(2)). Refining against inter-subunit paramagnetic NMR data, we show that free K63-Ub(2) exists as a dynamic ensemble comprising multiple closed and open quaternary states. The quaternary dynamics enables K63-Ub(2) to be specifically recognized in a variety of signaling pathways. When binding to a target protein, one of the preexisting quaternary states is selected and stabilized. A point mutation that shifts the equilibrium between the different states modulates the binding affinities towards K63-Ub(2) ligands. This conformational selection mechanism at the quaternary level may be used by polyubiquitins of different lengths and linkages for target recognition. DOI: 10.7554/eLife.05767.001
WOS标题词	Science & Technology ; Life Sciences & Biomedicine
类目[WOS]	Biology
研究领域[WOS]	Life Sciences & Biomedicine - Other Topics
关键词[WOS]	PARAMAGNETIC RELAXATION ENHANCEMENT ; 63-LINKED POLYUBIQUITIN CHAINS ; NF-KAPPA-B ; STRUCTURAL BASIS ; BIOLOGICAL MACROMOLECULES ; NMR ; BINDING ; TETRAUBIQUITIN ; REVEALS ; DIUBIQUITIN
收录类别	SCI
语种	英语
WOS记录号	WOS:000374251300001
内容类型	期刊论文
源URL	[http://ir.wipm.ac.cn/handle/112942/9323]
专题	武汉物理与数学研究所_磁共振应用研究部
作者单位	1.Chinese Acad Sci, Wuhan Inst Phys & Math, State Key Lab Magnet Resonance & Atom Mol Phys, CAS Key Lab Magnet Resonance Biol Syst, Wuhan, Peoples R China 2.Zhejiang Univ, Sch Med, Dept Pharmacol, Hangzhou 310003, Zhejiang, Peoples R China 3.Zhejiang Univ, Sch Med, Inst Neurosci, Hangzhou 310003, Zhejiang, Peoples R China
推荐引用方式 GB/T 7714	Liu, Zhu,Gong, Zhou,Jiang, Wen-Xue,et al. Lys63-linked ubiquitin chain adopts multiple conformational states for specific target recognition[J]. ELIFE,2015,4.
APA	Liu, Zhu.,Gong, Zhou.,Jiang, Wen-Xue.,Yang, Ju.,Zhu, Wen-Kai.,...&Tang, Chun.(2015).Lys63-linked ubiquitin chain adopts multiple conformational states for specific target recognition.ELIFE,4.
MLA	Liu, Zhu,et al."Lys63-linked ubiquitin chain adopts multiple conformational states for specific target recognition".ELIFE 4(2015).