An Unusual Dehydratase Acting on Glycerate and a Ketoreducatse Stereoselectively Reducing alpha-Ketone in Polyketide Starter Unit Biosynthesis

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	An Unusual Dehydratase Acting on Glycerate and a Ketoreducatse Stereoselectively Reducing alpha-Ketone in Polyketide Starter Unit Biosynthesis
	He HY(贺海燕)1; Yuan H(袁华)1; Tang MC(唐满成)1; Tang GL(唐功利)1
刊名	Angew. Chem.-Int. Edit.
	2014
卷号	53 期号:42 页码:11315-11319
其他题名	聚酮起始单元生物合成中一个新的脱水酶作用于甘油底物及酮基还原酶立体选择性还原a-酮基
通讯作者	唐功利
英文摘要	Polyketide synthases (PKSs) usually employ a ketoreductase (KR) to catalyze the reduction of a -keto group, followed by a dehydratase (DH) that drives the dehydration to form a double bond between the - and -carbon atoms. Herein, a DH-KR involved in FR901464 biosynthesis was characterized: DH* acts on glyceryl-S-acyl carrier protein (ACP) to yield ACP-linked pyruvate; subsequently KR* reduces -ketone that yields L-lactyl-S-ACP as starter unit for polyketide biosynthesis. Genetic and biochemical evidence was found to support a similar pathway that is involved in the biosynthesis of lankacidins. These results not only identified new PKS domains acting on different substrates, but also provided additional options for engineering the PKS starter pathway or biocatalysis.
学科主题	生命有机化学
收录类别	SCI
原文出处	http://dx.doi.org/10.1002/anie.201406602
语种	英语
内容类型	期刊论文
源URL	[http://ir.sioc.ac.cn/handle/331003/38960]
专题	上海有机化学研究所_生命有机化学国家重点实验室
作者单位	1.中科院上海有机化学研究所 2.上海生物制造技术协同创新中心
推荐引用方式 GB/T 7714	He HY,Yuan H,Tang MC,et al. An Unusual Dehydratase Acting on Glycerate and a Ketoreducatse Stereoselectively Reducing alpha-Ketone in Polyketide Starter Unit Biosynthesis[J]. Angew. Chem.-Int. Edit.,2014,53(42):11315-11319.
APA	贺海燕,袁华,唐满成,&唐功利.(2014).An Unusual Dehydratase Acting on Glycerate and a Ketoreducatse Stereoselectively Reducing alpha-Ketone in Polyketide Starter Unit Biosynthesis.Angew. Chem.-Int. Edit.,53(42),11315-11319.
MLA	贺海燕,et al."An Unusual Dehydratase Acting on Glycerate and a Ketoreducatse Stereoselectively Reducing alpha-Ketone in Polyketide Starter Unit Biosynthesis".Angew. Chem.-Int. Edit. 53.42(2014):11315-11319.