In situ observation of C-60(C(COOH)(2))(2) interacting with living cells using fluorescence microscopy

	In situ observation of C-60(C(COOH)(2))(2) interacting with living cells using fluorescence microscopy
	Ye C(叶昶); Chen CY(陈春英); Chen Z(陈真); Meng H(孟幻); Li X(李湘); Yuan H(袁慧); Xing GM(邢更妹); Zhao F(赵峰); Zhao YL(赵宇亮); Chai ZF(柴之芳)
刊名	CHINESE SCIENCE BULLETIN
	2006
卷号	51 期号:9 页码:1060-1064
关键词	fluorescence microscopy real time imaging fullerene derivative C-60(C(COOH)(2))(2) cross cellular membrane fluorescence labeling
通讯作者	Chinese Acad Sci, Lab Bioenvironm Hlth Sci Nanoscale Mat, Inst High Energy Phys, Beijing 100049, Peoples R China ; Chinese Acad Sci, Inst Chem, Key Lab Mol Nanostruct & Nanotechnol, Beijing 100080, Peoples R China ; Natl Ctr Nanosci & Technol, Beijing 100080, Peoples R China ; Chinese Acad Sci, Inst Biophys, Beijing 100101, Peoples R China
英文摘要	The interactions between nanoparticles and living cells were investigated by an imaging technique of fluorescence microscopy. For this purpose, the C-60 derivative C-60(C(COOH)(2))(2), a therapeutic agent for degeneration diseases of central nervous system, was synthesized, purified and characterized. Its interaction with the living cell and penetration of the cellular membrane were in situ studied using the real time imaging technique, and its potential cytotoxicity was also examined by flow cytometry. The results indicate that C-60(C(COOH)(2))(2) can easily enter cells, and is mainly located in cytoplasm by fluorescein labeling. Furthermore, C-60(C(COOH)(2))(2) can carry the molecule that cannot cross cellular membranes into cells, because fluorescein compound itself cannot enter the cell or adhere to membrane. At concentrations ranging from 1 x 10(-2) to 1 x 10(2) mg/L, C-60(C(COOH)(2))(2) does not show any detectable cytotoxicity.
学科主题	Science & Technology - Other Topics
类目[WOS]	Multidisciplinary Sciences
研究领域[WOS]	Science & Technology - Other Topics
原文出处	SCI
语种	英语
WOS记录号	WOS:000238882500007
内容类型	期刊论文
源URL	[http://ir.ihep.ac.cn/handle/311005/238976]
专题	高能物理研究所_院士高能物理研究所_理论物理室高能物理研究所_多学科研究中心
作者单位	中国科学院高能物理研究所
推荐引用方式 GB/T 7714	Ye C,Chen CY,Chen Z,et al. In situ observation of C-60(C(COOH)(2))(2) interacting with living cells using fluorescence microscopy[J]. CHINESE SCIENCE BULLETIN,2006,51(9):1060-1064.
APA	叶昶.,陈春英.,陈真.,孟幻.,李湘.,...&Wei, TT.(2006).In situ observation of C-60(C(COOH)(2))(2) interacting with living cells using fluorescence microscopy.CHINESE SCIENCE BULLETIN,51(9),1060-1064.
MLA	叶昶,et al."In situ observation of C-60(C(COOH)(2))(2) interacting with living cells using fluorescence microscopy".CHINESE SCIENCE BULLETIN 51.9(2006):1060-1064.