An improved method for analysis of distribution of metalloproteins in human liver cytosol by synchrotron radiation X-ray fluorescence

	An improved method for analysis of distribution of metalloproteins in human liver cytosol by synchrotron radiation X-ray fluorescence
	Gao YX(高愈希); Chen CY(陈春英); Zhao JJ(赵九江); Zhang PQ(章佩群); Chai ZF(柴之芳); He W(何伟); Huang YY(黄宇营); Gao, YX; Chen, CY; Zhao, JJ
刊名	CHINESE JOURNAL OF ANALYTICAL CHEMISTRY
	2003
卷号	31 期号:4 页码:395-398
关键词	synchrotron radiation X-ray fluorescence analysis metalloprotein electrophoretic separation
其他题名	一种改进的同步辐射X射线荧光原位分析人肝细胞胞质溶胶内金属蛋白分布的方法
通讯作者	Chinese Acad Sci, Inst High Energy Phys, Lab Nucl Analyt Tech, Beijing 100039, Peoples R China ; Chinese Acad Sci, Inst High Energy Phys, Synchrotron Radiat Lab, Beijing 100039, Peoples R China
英文摘要	A method for the analysis of trace elements in protein bands with synchrotron radiation X-ray fluorescence (SRXFR) after electrophoresis separation was improved. Through a step of drying the gel before determination, the continues background mainly originated from the Compton scattering of X-ray upon the dry gel matrix was reduced to about one tenth of that upon the wet gel. Trace elements lighter than zinc in protein bands can be determined simultaneously. The method could be used for speciation of trace elements in biological sample.. Applying the improved procedure to investigate the distribution of metalloproteins in human liver cytosol,. six Zn-containing proteins with molecular weights(MWs) of 17.5, 20.5, 27, 35, 55, and 6 3 kdalton respectively, and four Fe-containing proteins with MWs of 20, 23, 43, and 83.5 kdalton respectively can be detected.. The 63 kdalton Zn-containing protein is the dominant form of zinc in the sample.
学科主题	Chemistry
类目[WOS]	Chemistry, Analytical
研究领域[WOS]	Chemistry
原文出处	SCI
语种	英语
WOS记录号	WOS:000182746400003
内容类型	期刊论文
源URL	[http://ir.ihep.ac.cn/handle/311005/237306]
专题	高能物理研究所_院士中国科学院高能物理研究所_中国散裂中子源
作者单位	中国科学院高能物理研究所
推荐引用方式 GB/T 7714	Gao YX,Chen CY,Zhao JJ,et al. An improved method for analysis of distribution of metalloproteins in human liver cytosol by synchrotron radiation X-ray fluorescence[J]. CHINESE JOURNAL OF ANALYTICAL CHEMISTRY,2003,31(4):395-398.
APA	高愈希.,陈春英.,赵九江.,章佩群.,柴之芳.,...&Huang, YY.(2003).An improved method for analysis of distribution of metalloproteins in human liver cytosol by synchrotron radiation X-ray fluorescence.CHINESE JOURNAL OF ANALYTICAL CHEMISTRY,31(4),395-398.
MLA	高愈希,et al."An improved method for analysis of distribution of metalloproteins in human liver cytosol by synchrotron radiation X-ray fluorescence".CHINESE JOURNAL OF ANALYTICAL CHEMISTRY 31.4(2003):395-398.