Co-occurrence of Methanosarcina mazei and Geobacteraceae in an iron (III)-reducing enrichment culture

CORC > 烟台海岸带研究所 > 中国科学院烟台海岸带研究所 > 海岸带生物学与生物资源利用重点实验室 > 海岸带生物学与生物资源利用所重点实验室

	Co-occurrence of Methanosarcina mazei and Geobacteraceae in an iron (III)-reducing enrichment culture
	Zheng, Shiling 1,2; Zhang, Hongxia 1,2,3; Li, Ying 1,2,3; Zhang, Hua1 ; Wang, Oumei 4; Zhang, Jun 5; Liu, Fanghua1,2
刊名	FRONTIERS IN MICROBIOLOGY
	2015-09-08
卷号	6 页码:941
关键词	co-occurrence Methanosarcina mazei Geobacteraceae direct interspecies electron transfer (DIET) iron(III)-reducing microorganisms
ISSN号	1664-302X
通讯作者	Wang, OM (reprint author), Binzhou Med Univ, Key Lab Genet Hearing Disorders Shandong, 346 Guanhai Rd, Yantai 264003, Shandong, Peoples R China. ziou79@hotmail.com ; fhliu@yic.ac.cn
产权排序	[Zheng, Shiling; Zhang, Hongxia; Li, Ying; Zhang, Hua; Liu, Fanghua] Chinese Acad Sci, Key Lab Coastal Environm Proc & Ecol Remediat, Yantai Inst Coastal Zone Res, Yantai, Peoples R China; [Zheng, Shiling; Zhang, Hongxia; Li, Ying; Liu, Fanghua] Chinese Acad Sci, Key Lab Coastal Biol & Biol Resources Utilizat, Yantai Inst Coastal Zone Res, Yantai, Peoples R China; [Zhang, Hongxia; Li, Ying] Univ Chinese Acad Sci, Beijing, Peoples R China; [Wang, Oumei] Binzhou Med Univ, Key Lab Genet Hearing Disorders Shandong, Yantai 264003, Shandong, Peoples R China; [Zhang, Jun] Northwest Univ, Coll Life Sci, Xian, Peoples R China
中文摘要	Methanosaeta harundinacea and Methanosarcina barkeri, known as classic acetoclastic methanogens, are capable of directly accepting electrons from Geobacter metallireducens for the reduction of carbon dioxide to methane, having been revealed as direct interspecies electron transfer (DIET) in the laboratory co-cultures. However, whether their co occurrences are ubiquitous in the iron (Ill) reducing environments and the other species of acetoclastic methanogens such as Methanosarcina mazei are capable of DIET are still unknown. Instead of initiating the co-cultures with pure cultures, two-step cultivation was employed to selectively enrich iron (Ill) reducing microorganisms in a coastal gold mining river, Jiehe River, with rich iron content in the sediments. First, iron (III) reducers including Geobacteraceae were successfully enriched by 3-months successive culture on amorphous Fe(III) oxides as electron acceptor and acetate as electron donor. High throughput Illumina sequencing, terminal restriction fragment length polymorphism (T-RFLP) and clone library analysis based on 16S rRNA genes revealed that the enrichment cultures actively contained the bacteria belong to Geobacteraceae and Bacilli, exclusively dominated by the archaea belong to Methanosarcinaceae. Second, the enrichment cultures including methanogens and Geobacteraceae were transferred with ethanol as alternative electron donor. Remarkably, aggregates were successively formed in the enrichments after three transfers. The results revealed by RNA-based analysis demonstrate that the co-occurrence of Methanosarcina mazei and Geobacteraceae in an iron (Ill) reducing enrichment culture. Furthermore, the aggregates, as close physical contact, formed in the enrichment culture, indicate that DIET could be a possible option for interspecies electron transfer in the aggregates.
英文摘要	Methanosaeta harundinacea and Methanosarcina barkeri, known as classic acetoclastic methanogens, are capable of directly accepting electrons from Geobacter metallireducens for the reduction of carbon dioxide to methane, having been revealed as direct interspecies electron transfer (DIET) in the laboratory co-cultures. However, whether their co occurrences are ubiquitous in the iron (Ill) reducing environments and the other species of acetoclastic methanogens such as Methanosarcina mazei are capable of DIET are still unknown. Instead of initiating the co-cultures with pure cultures, two-step cultivation was employed to selectively enrich iron (Ill) reducing microorganisms in a coastal gold mining river, Jiehe River, with rich iron content in the sediments. First, iron (III) reducers including Geobacteraceae were successfully enriched by 3-months successive culture on amorphous Fe(III) oxides as electron acceptor and acetate as electron donor. High throughput Illumina sequencing, terminal restriction fragment length polymorphism (T-RFLP) and clone library analysis based on 16S rRNA genes revealed that the enrichment cultures actively contained the bacteria belong to Geobacteraceae and Bacilli, exclusively dominated by the archaea belong to Methanosarcinaceae. Second, the enrichment cultures including methanogens and Geobacteraceae were transferred with ethanol as alternative electron donor. Remarkably, aggregates were successively formed in the enrichments after three transfers. The results revealed by RNA-based analysis demonstrate that the co-occurrence of Methanosarcina mazei and Geobacteraceae in an iron (Ill) reducing enrichment culture. Furthermore, the aggregates, as close physical contact, formed in the enrichment culture, indicate that DIET could be a possible option for interspecies electron transfer in the aggregates.
学科主题	Microbiology
研究领域[WOS]	Microbiology
关键词[WOS]	INTERSPECIES ELECTRON-TRANSFER ; GRADIENT GEL-ELECTROPHORESIS ; SPORE-FORMING BACTERIUM ; RICE FIELD SOIL ; PADDY SOIL ; METHANE PRODUCTION ; FERRIC IRON ; REDUCING MICROORGANISMS ; ARCHAEAL COMMUNITY ; SP-NOV
收录类别	SCI
语种	英语
WOS记录号	WOS:000361013800001
内容类型	期刊论文
源URL	[http://ir.yic.ac.cn/handle/133337/13806]
专题	烟台海岸带研究所_海岸带生物学与生物资源利用所重点实验室
作者单位	1.Chinese Acad Sci, Key Lab Coastal Environm Proc & Ecol Remediat, Yantai Inst Coastal Zone Res, Yantai, Peoples R China 2.Chinese Acad Sci, Key Lab Coastal Biol & Biol Resources Utilizat, Yantai Inst Coastal Zone Res, Yantai, Peoples R China 3.Univ Chinese Acad Sci, Beijing, Peoples R China 4.Binzhou Med Univ, Key Lab Genet Hearing Disorders Shandong, Yantai 264003, Shandong, Peoples R China 5.Northwest Univ, Coll Life Sci, Xian, Peoples R China
推荐引用方式 GB/T 7714	Zheng, Shiling,Zhang, Hongxia,Li, Ying,et al. Co-occurrence of Methanosarcina mazei and Geobacteraceae in an iron (III)-reducing enrichment culture[J]. FRONTIERS IN MICROBIOLOGY,2015,6:941.
APA	Zheng, Shiling.,Zhang, Hongxia.,Li, Ying.,Zhang, Hua.,Wang, Oumei.,...&Liu, Fanghua.(2015).Co-occurrence of Methanosarcina mazei and Geobacteraceae in an iron (III)-reducing enrichment culture.FRONTIERS IN MICROBIOLOGY,6,941.
MLA	Zheng, Shiling,et al."Co-occurrence of Methanosarcina mazei and Geobacteraceae in an iron (III)-reducing enrichment culture".FRONTIERS IN MICROBIOLOGY 6(2015):941.