Label-free aptamer biosensor for thrombin detection based on functionalized graphene nanocomposites

	Label-free aptamer biosensor for thrombin detection based on functionalized graphene nanocomposites
	Wang,Qingqing; Zhou,Zhixue; Zhai,Yanling; Zhang,Lingling; Hong,Wei; Zhang,Zhiquan; Dong,Shaojun
刊名	talanta
	2015
卷号	141 页码:247-252
关键词	SURFACE-PLASMON RESONANCE GOLD NANOPARTICLES ELECTROCHEMICAL APTASENSOR ULTRASENSITIVE DETECTION SENSING PLATFORM ENERGY-TRANSFER OXIDE ELECTRODE AMPLIFICATION PROTEIN
通讯作者	zhang,zq
英文摘要	a label-free and amplified electrochemical impedimetric aptasensor based on functionalized graphene nanocomposites (rgo-aunps) was developed for the detection of thrombin, which played a vital role in thrombosis and hemostasis. the thiolated aptamer and dithiothreitol (tba15-dtt) were firstly immobilized on the gold electrode to capture the thrombin molecules, and then aptamer functionalized graphene nanocomposites (rgo-tba29) were used to fabricate a sandwich sensing platform for amplifying the impedimetric signals. as numerous negative charges of tba29 on the electrode repelled to the [fe(cn)(6)](4-/3-) anions, resulting in an obvious amplified charge-transfer resistance (r-ct) signal. the r-ct increase was linearly proportional to the thrombin concentration from 0.3 to 50 nm and a detection limit of 0.01 nm thrombin was achieved. in addition, graphene could also be labeled with other probes via electrostatic or pi-pi stacking interactions to produce signals, therefore different detection methods expanding wide application could be used in this model. (c) 2015 published by elsevier b.v.
收录类别	SCI
语种	英语
公开日期	2016-05-31
内容类型	期刊论文
源URL	[http://ir.ciac.jl.cn/handle/322003/67904]
专题	长春应用化学研究所_长春应用化学研究所知识产出_期刊论文
推荐引用方式 GB/T 7714	Wang,Qingqing,Zhou,Zhixue,Zhai,Yanling,et al. Label-free aptamer biosensor for thrombin detection based on functionalized graphene nanocomposites[J]. talanta,2015,141:247-252.
APA	Wang,Qingqing.,Zhou,Zhixue.,Zhai,Yanling.,Zhang,Lingling.,Hong,Wei.,...&Dong,Shaojun.(2015).Label-free aptamer biosensor for thrombin detection based on functionalized graphene nanocomposites.talanta,141,247-252.
MLA	Wang,Qingqing,et al."Label-free aptamer biosensor for thrombin detection based on functionalized graphene nanocomposites".talanta 141(2015):247-252.