Ultrasensitive time-resolved microplate fluorescence immunoassay for bisphenol A using a system composed on gold nanoparticles and a europium(III)-labeled streptavidin tracer

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	Ultrasensitive time-resolved microplate fluorescence immunoassay for bisphenol A using a system composed on gold nanoparticles and a europium(III)-labeled streptavidin tracer
	Du, Lingyun ; Zhang, Chunyan ; Wang, Longjun ; Liu, Guofu ; Zhang, Yuanfu ; Wang, Shuhao
刊名	MICROCHIMICA ACTA
	2015
卷号	182 期号:42433 页码:539-545
关键词	TANDEM MASS-SPECTROMETRY LIQUID-LIQUID MICROEXTRACTION EXPOSURE RISK EXTRACTION EXPRESSION PROTEINS QUECHERS CANCER SIGNAL
ISSN号	0026-3672
中文摘要	We demonstrate an ultrasensitive time-resolved fluorescence immunoassay for bisphenol A (BPA) where gold nanoparticles (Au-NPs) were modified with anti-BPA antibody and thiolated dsDNA-biotin acting as a signal amplifier. In a competitive reaction, the analyte (BPA) competes with immobilized BPA-ovalbumin conjugate on the surface of microtiter plates to bind to the anti-BPA antibodies on the surface of the Au-NPs. In the next step, a Eu(III)-labeled streptavidin is added to link to the SH-dsDNA-biotin as a tracer. Fluorescence is amplified via both the Au-NPs and the biotin-streptavidin systems, and its intensity is measured in a time-resolved fluorescence immunoassay. The linear range is from 1.0 fga (TM) mL(-1) to 1.0 nga (TM) mL(-1), and the detection limit is 0.3 fg mL(-1), respectively. The method was applied to the determination of BPA in spiked water sample, with recoveries ranging from 90.2 to 106.4 %.
收录类别	SCI
原文出处	10.1007/s00604-014-1356-9
语种	英语
公开日期	2016-05-12
内容类型	期刊论文
源URL	[http://ir.xtbg.org.cn/handle/353005/9812]
专题	西双版纳热带植物园_文献共享
推荐引用方式 GB/T 7714	Du, Lingyun,Zhang, Chunyan,Wang, Longjun,et al. Ultrasensitive time-resolved microplate fluorescence immunoassay for bisphenol A using a system composed on gold nanoparticles and a europium(III)-labeled streptavidin tracer[J]. MICROCHIMICA ACTA,2015,182(42433):539-545.
APA	Du, Lingyun,Zhang, Chunyan,Wang, Longjun,Liu, Guofu,Zhang, Yuanfu,&Wang, Shuhao.(2015).Ultrasensitive time-resolved microplate fluorescence immunoassay for bisphenol A using a system composed on gold nanoparticles and a europium(III)-labeled streptavidin tracer.MICROCHIMICA ACTA,182(42433),539-545.
MLA	Du, Lingyun,et al."Ultrasensitive time-resolved microplate fluorescence immunoassay for bisphenol A using a system composed on gold nanoparticles and a europium(III)-labeled streptavidin tracer".MICROCHIMICA ACTA 182.42433(2015):539-545.