Integration of bacterial expansin-like proteins into cellulosome promotes the cellulose degradation | |
Chen, Chao1,2,3,4; Cui, Zhenling1,2,3,4; Song, Xiangfei2,3; Liu, Ya-Jun1,2,3; Cui, Qiu1,2,3; Feng, Yingang1,2,3 | |
刊名 | APPLIED MICROBIOLOGY AND BIOTECHNOLOGY
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2016-03-01 | |
卷号 | 100期号:5页码:2203-2212 |
关键词 | Expansin Cellulosome Cellulose hydrolysis Dockerin Clostridium clariflavum |
英文摘要 | Cellulosomes are multi-enzyme complexes assembled by cellulases and hemicellulases through dockerin-cohesin interactions, which are the most efficient system for the degradation of lignocellulosic resources in nature. Recent genomic analysis of a cellulosome-producing anaerobe Clostridium clariflavum DSM 19732 revealed that two expansin-like proteins, Clocl_1298 and Clocl_1862, contain a dockerin module, which suggests that they are components of the cellulosome. Bacterial expansin-like proteins do not have hydrolytic activities, but can facilitate the degradation of cellulosic biomass via synergistic effects with cellulases. In this study, the synergistic effect of the expansin-like proteins with both native and designer cellulosomes was investigated. The free expansin-like proteins, including expansin-like domains of Clocl_1298 and Clocl_1862, as well as a well-studied bacterial expansin-like protein BsEXLX1 from Bacillus subtilis, promoted the cellulose degradation by native cellulosomes, indicating the cellulosomal expansin-like proteins have the synergistic function. When they were integrated into a trivalent designer cellulosome, the synergistic effect was further amplified. The sequence and structure analyses indicated that these cellulosomal expansin-like proteins share the conserved functional mechanism with other bacterial expansin-like proteins. These results indicated that non-catalytic expansin-like proteins in the cellulosome can enhance the activity of the cellulosome in lignocellulose degradation. The involvement of functional expansin-like proteins in the cellulosome also implies new physiological functions of bacterial expansin-like proteins and cellulosomes. |
WOS标题词 | Science & Technology ; Life Sciences & Biomedicine |
类目[WOS] | Biotechnology & Applied Microbiology |
研究领域[WOS] | Biotechnology & Applied Microbiology |
关键词[WOS] | CLOSTRIDIUM-THERMOCELLUM CELLULOSOME ; BACILLUS-SUBTILIS ; BINDING CHARACTERISTICS ; GENE-EXPRESSION ; CELLULOLYTICUM ; HYDROLYSIS ; IDENTIFICATION ; CLARIFLAVUM ; COMPONENTS ; INHIBITOR |
收录类别 | SCI |
语种 | 英语 |
WOS记录号 | WOS:000371243500016 |
内容类型 | 期刊论文 |
源URL | [http://ir.qibebt.ac.cn/handle/337004/8052] ![]() |
专题 | 青岛生物能源与过程研究所_代谢物组学团队 |
作者单位 | 1.Chinese Acad Sci, Qingdao Inst Bioenergy & Bioproc Technol, Qingdao Engn Lab Single Cell Oil, 189 Songling Rd, Qingdao 266101, Shandong, Peoples R China 2.Chinese Acad Sci, Qingdao Inst Bioenergy & Bioproc Technol, Shandong Prov Key Lab Energy Genet, 189 Songling Rd, Qingdao 266101, Shandong, Peoples R China 3.Chinese Acad Sci, CAS Key Lab Biofuels, Qingdao Inst Bioenergy & Bioproc Technol, 189 Songling Rd, Qingdao 266101, Shandong, Peoples R China 4.Univ Chinese Acad Sci, Beijing 100049, Peoples R China |
推荐引用方式 GB/T 7714 | Chen, Chao,Cui, Zhenling,Song, Xiangfei,et al. Integration of bacterial expansin-like proteins into cellulosome promotes the cellulose degradation[J]. APPLIED MICROBIOLOGY AND BIOTECHNOLOGY,2016,100(5):2203-2212. |
APA | Chen, Chao,Cui, Zhenling,Song, Xiangfei,Liu, Ya-Jun,Cui, Qiu,&Feng, Yingang.(2016).Integration of bacterial expansin-like proteins into cellulosome promotes the cellulose degradation.APPLIED MICROBIOLOGY AND BIOTECHNOLOGY,100(5),2203-2212. |
MLA | Chen, Chao,et al."Integration of bacterial expansin-like proteins into cellulosome promotes the cellulose degradation".APPLIED MICROBIOLOGY AND BIOTECHNOLOGY 100.5(2016):2203-2212. |
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