Utilization of deep eutectic solvents as novel mobile phase additives for improving the separation of bioactive quaternary alkaloids | |
Tan T(谭婷)1,3; Zhang ML(张明亮)3; Wan YQ(万益群)1,2; Qiu HD(邱洪灯)3 | |
刊名 | Talanta |
2016 | |
卷号 | 149页码:85-90 |
关键词 | Deep eutectic solvents Quaternary alkaloids Mobile phase additives Retention mechanism Choline chloride HPLC |
ISSN号 | 0039-9140 |
英文摘要 | Deep eutectic solvents (DESs) were used as novel mobile phase additives to improve chromatographic separation of four quaternary alkaloids including coptisine chloride, sanguinarine, berberine chloride and chelerythrine on a C18 column. DESs as a new class of ionic liquids are renewably sourced, environmentally benign, low cost and easy to prepare. Seven DESs were obtained by mixing different hydrogen acceptors and hydrogen-bond donors. The effects of organic solvents, the concentration of DESs, the types of DESs and the pH values of the buffer solution on the separation of the analytes were investigated. The composition of acetonitrile and 1.0% deep eutectic solvents aqueous solution (pH 3.3, adjusted with hydrochloric acid) in a 32:68 (v/v) ratio was used as optimized mobile phase, with which four quaternary alkaloids were well separated. When a small amount of DESs was added in the mobile phase for the separation of alkaloids on the C18 column, noticeable improvements were distinctly observed such as decreasing peak tailing and improving resolution. The separation mechanism mediated by DESs as mobile phase additives can be attributed to combined effect of both hydrogen acceptors and hydrogen-bond donors. For example, choline chloride can effectively cover the residual silanols on silica surface and ethylene glycol can reduce the retention time of analytes. The proposed method has been applied to determine BerbC in Lanqin Chinese herbal oral solution and BerbC tablet. Utilization of DESs in mobile phase can efficiently improve separation and selectivity of analytes from complex samples. |
学科主题 | 分析化学与药物化学 |
收录类别 | SCI |
资助信息 | the “Hundred Talents Program” of the Chinese Academy of Sciences;the Science and Technology Program of Jiangxi Province (20141BBG70093);the Open Project Program of State Key Laboratory of Food Science and Technology, Nanchang University (No. SKLF-KF-201404) |
语种 | 英语 |
WOS记录号 | WOS:000368866900011 |
内容类型 | 期刊论文 |
源URL | [http://210.77.64.217/handle/362003/19703] |
专题 | 兰州化学物理研究所_中科院西北特色植物资源化学重点实验室/甘肃省天然药物重点实验室 |
作者单位 | 1.State Key Laboratory of Food Science and Technology, Nanchang University, Nanchang 330047, China 2.School of Chemistry, Nanchang University, Nanchang 330031, China 3.Key Laboratory of Chemistry of Northwestern Plant Resources and Key Laboratory for Natural Medicine of Gansu Province, Lanzhou Institute of Chemical Physics, Chinese Academy of Sciences, Lanzhou 730000, China |
推荐引用方式 GB/T 7714 | Tan T,Zhang ML,Wan YQ,et al. Utilization of deep eutectic solvents as novel mobile phase additives for improving the separation of bioactive quaternary alkaloids[J]. Talanta,2016,149:85-90. |
APA | Tan T,Zhang ML,Wan YQ,&Qiu HD.(2016).Utilization of deep eutectic solvents as novel mobile phase additives for improving the separation of bioactive quaternary alkaloids.Talanta,149,85-90. |
MLA | Tan T,et al."Utilization of deep eutectic solvents as novel mobile phase additives for improving the separation of bioactive quaternary alkaloids".Talanta 149(2016):85-90. |
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