Functional and structural properties of a novel cellulosome-like multienzyme complex: efficient glycoside hydrolysis of water-insoluble 7-xylosyl-10-deacetylpaclitaxel

	Functional and structural properties of a novel cellulosome-like multienzyme complex: efficient glycoside hydrolysis of water-insoluble 7-xylosyl-10-deacetylpaclitaxel
	Dou, Tong-Yi 1,2; Luan, Hong-Wei 1; Ge, Guang-Bo 1; Dong, Ming-Ming 3; Zou, Han-Fa 3; He, Yu-Qi 1; Cui, Pan 1,2; Wang, Jia-Yue 1,2; Hao, Da-Cheng 4; Yang, Shi-Lin 5
刊名	scientific reports
	2015-09-08
卷号	5
英文摘要	cellulosome is a kind of multienzyme complex that displays high activity, selectivity, and stability. here, we report a novel, non-cellulolytic, cellulosome-like multienzyme complex that produced by the cellulosimicrobium cellulans wild-type strain f16 isolated from soil microflora. this multienzyme complex, with excellent catalytic efficiency of k(cat) 13.2 s(-1) to remove the c-7 xylosyl group from 7-xylosyl-10-deacetylpaclitaxel (10-daxp), has an outstanding tolerance against organic solvents and an excellent general stability, with the long half-life of 214 hours. this cellulosome-like multienzyme complex has a novel structure distinct from the well-documented ones. the key catalytic subunit responsible for the beta-xylosidase activity against 10-daxp is identified to be a novel protein, indicating a new glycoside hydrolase (gh) family. the pioneering work described here offers a novel nanoscale biocatalyst for the production of biofuels and chemicals from renewable plant-based natural resources.
WOS标题词	science & technology
类目[WOS]	multidisciplinary sciences
研究领域[WOS]	science & technology - other topics
关键词[WOS]	cellulolytic enzyme complex ; clostridium-thermocellum ; xylosidase ; biocatalysis ; organization ; hydrolases ; database ; taxanes ; barley
收录类别	SCI
语种	英语
WOS记录号	WOS:000360798000003
公开日期	2016-05-09
内容类型	期刊论文
源URL	[http://cas-ir.dicp.ac.cn/handle/321008/146512]
专题	大连化学物理研究所_中国科学院大连化学物理研究所
作者单位	1.Chinese Acad Sci, Dalian Inst Chem Phys, Lab Pharmaceut Resource Discovery, Dalian 116023, Peoples R China 2.Univ Chinese Acad Sci, Beijing 100049, Peoples R China 3.Chinese Acad Sci, Dalian Inst Chem Phys, Key Lab Separat Sci Analyt Chem, Natl Chromatog Res & Anal Ctr, Dalian 116023, Peoples R China 4.Dalian Jiaotong Univ, Sch Environm & Chem Engn, Inst Biotechnol, Dalian 116028, Peoples R China 5.Jiangxi Univ Tradit Chinese Med, Nanchang 330004, Peoples R China
推荐引用方式 GB/T 7714	Dou, Tong-Yi,Luan, Hong-Wei,Ge, Guang-Bo,et al. Functional and structural properties of a novel cellulosome-like multienzyme complex: efficient glycoside hydrolysis of water-insoluble 7-xylosyl-10-deacetylpaclitaxel[J]. scientific reports,2015,5.
APA	Dou, Tong-Yi.,Luan, Hong-Wei.,Ge, Guang-Bo.,Dong, Ming-Ming.,Zou, Han-Fa.,...&Yang, Ling.(2015).Functional and structural properties of a novel cellulosome-like multienzyme complex: efficient glycoside hydrolysis of water-insoluble 7-xylosyl-10-deacetylpaclitaxel.scientific reports,5.
MLA	Dou, Tong-Yi,et al."Functional and structural properties of a novel cellulosome-like multienzyme complex: efficient glycoside hydrolysis of water-insoluble 7-xylosyl-10-deacetylpaclitaxel".scientific reports 5(2015).