Growth and production of microencapsulated recombinant CHO in a stirred tank bioreactor

	Growth and production of microencapsulated recombinant CHO in a stirred tank bioreactor
	Wang, Yu 1,2; Zhang, Ying 1; Li, Na 1,2; Chen, Li 1,2; Zhang, Demeng 1,2; Sun, Dongsheng 1,2; Lv, Guojun 1; Yu, Weiting 1; Guo, Xin 1; Ma, Xiaojun 1
刊名	bioprocess and biosystems engineering
	2015-07-01
卷号	38 期号:7 页码:1303-1312
关键词	Cell culture Stirred tank bioreactor Microencapsulation Recombinant protein production
英文摘要	microencapsulation supplies cells with a three-dimensional microenvironment enhancing the metabolic activity, cell density and recombinant protein expression in a stirred tank bioreactor which is used widely to culture mammalian cells in many biochemical processes. in this paper, we address the growth and desmodus rotundus salivary plasminogen activator (dspa) production of recombinant cho (rcho) in a stirred tank bioreactor. cells were cultured using two different methods-in an unmicroencapsulated versus microencapsulated culture-and compared differences between them in terms of cell reproduction and dspa protein productivity. compared to the unmicroencapsulated rcho, microencapsulated cells got higher cell density and prolonged the plateau phase. microencapsulated rcho promoted dspa production, with a maximum rate that was 4.8 times higher than in unmicroencapsulated cells, and the accumulated production of dspa was 3.3 higher than in unmicroencapsulated cells. negative relationship was found between specific growth rate and dspa production capacity of unit cells. these findings will facilitate the methods for higher dspa production in stirred tank bioreactors.
WOS标题词	science & technology ; life sciences & biomedicine ; technology
类目[WOS]	biotechnology & applied microbiology ; engineering, chemical
研究领域[WOS]	biotechnology & applied microbiology ; engineering
关键词[WOS]	biopharmaceutical benchmarks ; hepatocyte encapsulation ; cell-culture ; microcapsules ; stability ; oxygen ; serum
收录类别	SCI
语种	英语
WOS记录号	WOS:000355940900008
公开日期	2016-05-09
内容类型	期刊论文
源URL	[http://cas-ir.dicp.ac.cn/handle/321008/146295]
专题	大连化学物理研究所_中国科学院大连化学物理研究所
作者单位	1.Chinese Acad Sci, Dalian Inst Chem Phys, Lab Biomed Mat Engn, Dalian 116023, Peoples R China 2.Univ Chinese Acad Sci, Grad Sch, Beijing 100039, Peoples R China
推荐引用方式 GB/T 7714	Wang, Yu,Zhang, Ying,Li, Na,et al. Growth and production of microencapsulated recombinant CHO in a stirred tank bioreactor[J]. bioprocess and biosystems engineering,2015,38(7):1303-1312.
APA	Wang, Yu.,Zhang, Ying.,Li, Na.,Chen, Li.,Zhang, Demeng.,...&Ma, Xiaojun.(2015).Growth and production of microencapsulated recombinant CHO in a stirred tank bioreactor.bioprocess and biosystems engineering,38(7),1303-1312.
MLA	Wang, Yu,et al."Growth and production of microencapsulated recombinant CHO in a stirred tank bioreactor".bioprocess and biosystems engineering 38.7(2015):1303-1312.