Higher-Order Human Telomeric G-Quadruplex DNA Metalloenzymes Enhance Enantioselectivity in the Diels-Alder Reaction

	Higher-Order Human Telomeric G-Quadruplex DNA Metalloenzymes Enhance Enantioselectivity in the Diels-Alder Reaction
	Li, Yinghao 1,2; Jia, Guoqing 1; Wang, Changhao 1,2; Cheng, Mingpan 1,2; Li, Can 1
刊名	chembiochem
	2015-03-02
卷号	16 期号:4 页码:618-624
关键词	Diels-Alder reaction DNA enantioselective catalysis G-quadruplexes human telomeric DNA metalloenzyme
英文摘要	short human telomeric (ht) dna sequences form single g-quadruplex (g(4)) units and exhibit structure-based stereocontrol for a series of reactions. however, for more biologically relevant higher-order ht g(4)-dnas (beyond a single g(4) unit), the catalytic performances are unknown. here, we found that higher-order ht g(4)-dna copper metalloenzymes (two or three g(4) units) afford remarkably higher enantioselectivity (>90% ee) and a five- to sixfold rate increase, compared to a single g(4) unit, for the diels-alder reaction. electron paramagnetic resonance (epr) and enzymatic kinetic studies revealed that the distinct catalytic function between single and higher-order g(4)-dna copper metalloenzymes can be attributed to different cu-ii coordination environments and substrate specificity. our finding suggests that, like protein enzymes and ribozymes, higher-order structural organization is crucial for g(4)-dna-based catalysis.
WOS标题词	science & technology ; life sciences & biomedicine
类目[WOS]	biochemistry & molecular biology ; chemistry, medicinal
研究领域[WOS]	biochemistry & molecular biology ; pharmacology & pharmacy
关键词[WOS]	asymmetric catalysis ; k+ solution ; circular-dichroism ; binding-site ; cd spectra ; ribozyme ; water ; polymorphism ; stability ; multimers
收录类别	SCI ; IC
语种	英语
WOS记录号	WOS:000350138700012
公开日期	2016-05-09
内容类型	期刊论文
源URL	[http://cas-ir.dicp.ac.cn/handle/321008/145978]
专题	大连化学物理研究所_中国科学院大连化学物理研究所
作者单位	1.Chinese Acad Sci, Dalian Inst Chem Phys, State Key Lab Catalysis, Dalian 116023, Peoples R China 2.Univ Chinese Acad Sci, Beijing 100049, Peoples R China
推荐引用方式 GB/T 7714	Li, Yinghao,Jia, Guoqing,Wang, Changhao,et al. Higher-Order Human Telomeric G-Quadruplex DNA Metalloenzymes Enhance Enantioselectivity in the Diels-Alder Reaction[J]. chembiochem,2015,16(4):618-624.
APA	Li, Yinghao,Jia, Guoqing,Wang, Changhao,Cheng, Mingpan,&Li, Can.(2015).Higher-Order Human Telomeric G-Quadruplex DNA Metalloenzymes Enhance Enantioselectivity in the Diels-Alder Reaction.chembiochem,16(4),618-624.
MLA	Li, Yinghao,et al."Higher-Order Human Telomeric G-Quadruplex DNA Metalloenzymes Enhance Enantioselectivity in the Diels-Alder Reaction".chembiochem 16.4(2015):618-624.