Serum metabolic profiling study of lung cancer using ultra high performance liquid chromatography/quadrupole time-of-flight mass spectrometry | |
Li, Yanjie1; Song, Xue2; Zhao, Xinjie1; Zou, Lijuan2; Xu, Guowang1 | |
刊名 | journal of chromatography b-analytical technologies in the biomedical and life sciences |
2014-09-01 | |
卷号 | 966页码:147-153 |
关键词 | Lung cancer Metabolomics Metabolic profiling Potential biomarker |
英文摘要 | lung cancer is currently the leading cause of cancer-related mortality worldwide. it is, therefore, important to enhance understanding and add a new auxiliary detection tool of lung cancer. in this work, serum metabolic characteristics of lung cancer were investigated with a non-targeted metabolomics method. the metabolic profiling of 23 patients with lung cancer and 23 healthy controls were analyzed using ultra high performance liquid chromatography/quadrupole time of flight mass spectrometry (uplc/q-tof ms). partial least squares discriminant analysis (pls-da) model of the metabolic data allowed the clear separation of the lung cancer patients from the healthy controls. in total, 27 differential metabolites were identified, which were mostly related to the perturbation of lipid metabolism, including choline, free fatty acids, lysophosphatidylcholines, etc. choline and linoleic acid were defined as one combinational biomarker using binary logistic regression, which was supported by the validation with a smaller sample-set (9 patients and 9 healthy controls). these findings show that lc/ms-based serum metabolic profiling has potential application in complementary identification of lung cancer patients, and could be a powerful tool for cancer research. (c) 2014 elsevier b.v. all rights reserved. |
WOS标题词 | science & technology ; life sciences & biomedicine ; physical sciences |
类目[WOS] | biochemical research methods ; chemistry, analytical |
研究领域[WOS] | biochemistry & molecular biology ; chemistry |
关键词[WOS] | human breast-cancer ; lipid-metabolism ; cigarette-smoking ; fatty-acids ; in-vitro ; metabonomics ; biomarkers ; signatures ; biology ; tobacco |
收录类别 | SCI |
语种 | 英语 |
WOS记录号 | WOS:000340323200018 |
公开日期 | 2016-05-09 |
内容类型 | 期刊论文 |
源URL | [http://cas-ir.dicp.ac.cn/handle/321008/145714] |
专题 | 大连化学物理研究所_中国科学院大连化学物理研究所 |
作者单位 | 1.Chinese Acad Sci, Dalian Inst Chem Phys, Key Lab Separat Sci Analyt Chem, Dalian 116023, Peoples R China 2.Dalian Med Univ, Dept Radiat Oncol, Affiliated Hosp 2, Dalian 116027, Peoples R China |
推荐引用方式 GB/T 7714 | Li, Yanjie,Song, Xue,Zhao, Xinjie,et al. Serum metabolic profiling study of lung cancer using ultra high performance liquid chromatography/quadrupole time-of-flight mass spectrometry[J]. journal of chromatography b-analytical technologies in the biomedical and life sciences,2014,966:147-153. |
APA | Li, Yanjie,Song, Xue,Zhao, Xinjie,Zou, Lijuan,&Xu, Guowang.(2014).Serum metabolic profiling study of lung cancer using ultra high performance liquid chromatography/quadrupole time-of-flight mass spectrometry.journal of chromatography b-analytical technologies in the biomedical and life sciences,966,147-153. |
MLA | Li, Yanjie,et al."Serum metabolic profiling study of lung cancer using ultra high performance liquid chromatography/quadrupole time-of-flight mass spectrometry".journal of chromatography b-analytical technologies in the biomedical and life sciences 966(2014):147-153. |
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