Analysis of the endogenous human serum peptides by on-line extraction with restricted-access material and HPLC-MS/MS identification

	Analysis of the endogenous human serum peptides by on-line extraction with restricted-access material and HPLC-MS/MS identification
	Hu, Lianghai 1,2; Boos, Karl-Siegfried 3; Ye, Mingliang 2; Zou, Hanfa 2
刊名	talanta
	2014-09-01
卷号	127 页码:191-195
关键词	Restrict-access materials Sample extraction Proteomics Serum peptides Two-dimensional
英文摘要	the selective extraction of endogenous serum peptides has been a challenge due to the high abundant proteins present in serum. here a simple on-line extraction of peptides from human serum using strong cation-exchange diol silica restricted-access materials (scx-ram) coupled with two-dimensional rp-rp liquid chromatography mass spectrometry was developed. the operation of the on-line extraction system is simple to use and does not need complex equipments. the two-dimensional rp-rp was proved to be orthogonal and efficient to separate peptides extracted from human serum. (c) 2014 elsevier b.v. all rights reserved.
WOS标题词	science & technology ; physical sciences
类目[WOS]	chemistry, analytical
研究领域[WOS]	chemistry
关键词[WOS]	multidimensional liquid-chromatography ; rp-rp-hplc ; sample preparation ; selective extraction ; mass-spectrometry ; biological-fluids ; lc-ms/ms ; peptidomics ; separation ; nanoparticles
收录类别	SCI
语种	英语
WOS记录号	WOS:000338620300026
公开日期	2016-05-09
内容类型	期刊论文
源URL	[http://cas-ir.dicp.ac.cn/handle/321008/145700]
专题	大连化学物理研究所_中国科学院大连化学物理研究所
作者单位	1.Jilin Univ, Sch Life Sci, Minist Educ, Key Lab Mol Enzymol & Engn, Changchun 130012, Peoples R China 2.Chinese Acad Sci, Dalian Inst Chem Phys, Key Lab Separat Sci Analyt Chem, Dalian 116023, Peoples R China 3.Univ Hosp Grosshadem, Inst Clin Chem, Lab BioSeparat, D-81366 Munich, Germany
推荐引用方式 GB/T 7714	Hu, Lianghai,Boos, Karl-Siegfried,Ye, Mingliang,et al. Analysis of the endogenous human serum peptides by on-line extraction with restricted-access material and HPLC-MS/MS identification[J]. talanta,2014,127:191-195.
APA	Hu, Lianghai,Boos, Karl-Siegfried,Ye, Mingliang,&Zou, Hanfa.(2014).Analysis of the endogenous human serum peptides by on-line extraction with restricted-access material and HPLC-MS/MS identification.talanta,127,191-195.
MLA	Hu, Lianghai,et al."Analysis of the endogenous human serum peptides by on-line extraction with restricted-access material and HPLC-MS/MS identification".talanta 127(2014):191-195.