Molecular magnetic resonance probe targeting VEGF165: preparation and in vitro and in vivo evaluation

	Molecular magnetic resonance probe targeting VEGF165: preparation and in vitro and in vivo evaluation
	You, Xiao-Guang 1,2; Tu, Rong 1,2; Peng, Ming-Li 3; Bai, Yu-Jie 1,2; Tan, Mingqian 4; Li, Han-Jian 1,2; Guan, Jing 5; Wen, Li-Jun 6
刊名	contrast media & molecular imaging
	2014-09-01
卷号	9 期号:5 页码:349-354
关键词	VEGF165 aptamer (USPIO) targeted molecular probe MRI liver cancer
英文摘要	a new method for imaging the tumor human vascular endothelial growth factor 165 (vegf 165) is presented. a magnetic resonance imaging (mri) probe was prepared by crosslinking ultrasmall superparamagnetic iron oxide (uspio) nanoparticles to the aptamer for tumor vascular endothelial growth factor 165 (vegf165-aptamer). the molecular probe was evaluated for its in vitro and in vivo activities toward vegf165. enzyme-linked immunosorbent assay showed that the vegf165-aptamer-uspio nanoparticles conjugate specifically binds to vegf165 in vitro. a cell proliferation test showed that vegf165-aptamer-uspio seems to block the proliferation of human umbilical vein endothelial cells induced by free vegf165, suggesting that vegf165 is an effective target of this molecular probe. in xenograft mice carrying liver cancer that expresses vegf165, t-2-weighted imaging of the tumor displayed marked negative enhancement 3h after the intravenous administration of vegf165-aptamer-uspio. the enhancement disappeared 6h after administration of the probe. these results suggest the targeted imaging effect of vegf165-aptamer-uspio probe in vivo for vegf165-expressing tumors. this is the first report of a targeted mri molecular probe based on uspio and vegf165-aptamer. copyright (c) 2014 john wiley & sons, ltd.
WOS标题词	science & technology ; life sciences & biomedicine
类目[WOS]	radiology, nuclear medicine & medical imaging
研究领域[WOS]	radiology, nuclear medicine & medical imaging
关键词[WOS]	endothelial growth-factor ; contrast agents ; anti-vegf ; cancer ; angiogenesis ; nanoparticles ; design
收录类别	SCI
语种	英语
WOS记录号	WOS:000343878700004
公开日期	2016-05-09
内容类型	期刊论文
源URL	[http://cas-ir.dicp.ac.cn/handle/321008/145654]
专题	大连化学物理研究所_中国科学院大连化学物理研究所
作者单位	1.Hainan Med Coll, Dept Radiol, Haikou 570102, Hainan Province, Peoples R China 2.Hainan Med Coll, Inst Canc, Haikou 570102, Hainan Province, Peoples R China 3.Northwest Univ, Xian 710068, Shanxi Province, Peoples R China 4.Chinese Acad Sci, Dalian Inst Chem Phys, Lab Biomed Mat Engn, Dalian 116023, Peoples R China 5.Zhunyi Med Coll Hop, Dept Radiol, Zhunyi City 564400, Guizhou Provinc, Peoples R China 6.Hainan Med Coll, Sch Pharm, Haikou 571101, Hainan Province, Peoples R China
推荐引用方式 GB/T 7714	You, Xiao-Guang,Tu, Rong,Peng, Ming-Li,et al. Molecular magnetic resonance probe targeting VEGF165: preparation and in vitro and in vivo evaluation[J]. contrast media & molecular imaging,2014,9(5):349-354.
APA	You, Xiao-Guang.,Tu, Rong.,Peng, Ming-Li.,Bai, Yu-Jie.,Tan, Mingqian.,...&Wen, Li-Jun.(2014).Molecular magnetic resonance probe targeting VEGF165: preparation and in vitro and in vivo evaluation.contrast media & molecular imaging,9(5),349-354.
MLA	You, Xiao-Guang,et al."Molecular magnetic resonance probe targeting VEGF165: preparation and in vitro and in vivo evaluation".contrast media & molecular imaging 9.5(2014):349-354.