Effect of Different Charged Liposomes on Transmembrane Incorporation Efficiency of the Staphylococcus Aureus Receptor Histidine Kinase AgrC

	Effect of Different Charged Liposomes on Transmembrane Incorporation Efficiency of the Staphylococcus Aureus Receptor Histidine Kinase AgrC
	Xiong Wen 1,2,3; Quan Chunshan 1,2,3; Wang Lina 4; Zhang Xuning 5; Zhao Jing 1,2,3; Zheng Wei 1,2,3; Fan Shengdi 1,2,3
刊名	chemical journal of chinese universities-chinese
	2014-09-10
卷号	35 期号:9 页码:1901-1907
关键词	Staphylococcus aureus AgrC Liposome Proteoliposome Membrane protein
英文摘要	agrc is a membrane-embedded histidine kinase in staphylococcus aureus that is thought to act as a sensor for the recognition of environmental signals and the transduction of signals into the cytoplasm so as to regulate and control a series of related pathogenic gene expression. however, for the complexity of cell membrane, it turns to be difficult to study agrc on bacterial cell membrane directly. many researches tried to take advantage of proteoliposome which could provide an approximate natural membrane environment and keep the protein activity to study the structure and function of membrane proteins. in order to build a transmembrane protein incorporation system with a relatively high incorporation efficiency, function activity and stability, various factors were considered, such as the impact of different charge polarity head on protein transmembrane incorporation efficiency. here, four kinds of different charged liposomes were prepared by changing the phospholipid components in liposome and a detergent-mediated method was used to reconstitute truncated agrc protein(agrc(tm6-7c)) into them. the results showed that negatively charged liposomes leaded to a higher incorporation efficiency compared with the positively charged one and about 60%-70% c-terminal cytoplasmic domain of agrc(tm6-7c) c protein reconstituted into negatively charged liposomes were outside-orientated which remained a highly kinase activity. furthermore, from circular dichroism detection result, we can draw a conclusion that liposome has certain protective effect on agrc(tm6-7c)-protein secondary structure and improve the thermal stability of the protein.
WOS标题词	science & technology ; physical sciences
类目[WOS]	chemistry, multidisciplinary
研究领域[WOS]	chemistry
关键词[WOS]	lipid-protein interactions ; membrane-proteins ; virulence ; perspective ; infections ; sensor
收录类别	SCI
语种	英语
WOS记录号	WOS:000343189300009
公开日期	2016-05-09
内容类型	期刊论文
源URL	[http://cas-ir.dicp.ac.cn/handle/321008/145470]
专题	大连化学物理研究所_中国科学院大连化学物理研究所
作者单位	1.Dalian Nationalities Univ, Key Lab Biotechnol & Resource Utilizat, State Ethn Affairs Commiss, Dalian 116600, Peoples R China 2.Dalian Nationalities Univ, Minist Educ, Dalian 116600, Peoples R China 3.Dalian Nationalities Univ, Dept Life Sci, Dalian 116600, Peoples R China 4.Chinese Acad Sci, Dalian Inst Chem Phys, Dalian 116023, Peoples R China 5.Dalian Polytech Univ, Sch Biol Engn, Dalian 116034, Peoples R China
推荐引用方式 GB/T 7714	Xiong Wen,Quan Chunshan,Wang Lina,et al. Effect of Different Charged Liposomes on Transmembrane Incorporation Efficiency of the Staphylococcus Aureus Receptor Histidine Kinase AgrC[J]. chemical journal of chinese universities-chinese,2014,35(9):1901-1907.
APA	Xiong Wen.,Quan Chunshan.,Wang Lina.,Zhang Xuning.,Zhao Jing.,...&Fan Shengdi.(2014).Effect of Different Charged Liposomes on Transmembrane Incorporation Efficiency of the Staphylococcus Aureus Receptor Histidine Kinase AgrC.chemical journal of chinese universities-chinese,35(9),1901-1907.
MLA	Xiong Wen,et al."Effect of Different Charged Liposomes on Transmembrane Incorporation Efficiency of the Staphylococcus Aureus Receptor Histidine Kinase AgrC".chemical journal of chinese universities-chinese 35.9(2014):1901-1907.