New insight into transmembrane topology of Staphylococcus aureus histidine kinase AgrC

	New insight into transmembrane topology of Staphylococcus aureus histidine kinase AgrC
	Wang, Lina 1; Quan, Chunshan 2,3; Xiong, Wen 2,3; Qu, Xiaojing 2,3; Fan, Shengdi 2,3; Hu, Wenzhong 2,3
刊名	biochimica et biophysica acta-biomembranes
	2014-03-01
卷号	1838 期号:3 页码:988-993
关键词	AgrC Topology GFP SCAM Staphylococcus aureus
英文摘要	staphylococcus aureus accessory gene regulator (agr) locus controls the expression of virulence factors through a classical two-component signal transduction system that consists of a receptor histidine protein kinase agrc and a cytoplasmic response regulator agra. an autoinducing peptide (aip) encoded by agr locus activates agrc, which transduces extracellular signals into the cytoplasm. despite extensive investigations to identify agrc-aip interaction sites, precise signal recognition mechanisms remain unknown. this study aims to clarify the membrane topology of agrc by applying the green fluorescent protein (gfp) fusion technique and the substituted cysteine accessibility method (scam). however, our findings were inconsistent with profile obtained previously by alkaline phosphatase. we report the topology of agrc shows seven transmembrane segments, a periplasmic n-terminus, and a cytoplasmic c-terminus. (c) 2013 elsevier b.v. all rights reserved.
WOS标题词	science & technology ; life sciences & biomedicine
类目[WOS]	biochemistry & molecular biology ; biophysics
研究领域[WOS]	biochemistry & molecular biology ; biophysics
关键词[WOS]	cysteine accessibility method ; membrane-protein topogenesis ; virulence genes ; regulatory rna ; united-states ; rf cloning ; receptor ; prediction ; recognition ; identification
收录类别	SCI
语种	英语
WOS记录号	WOS:000331661800029
公开日期	2016-05-09
内容类型	期刊论文
源URL	[http://cas-ir.dicp.ac.cn/handle/321008/145426]
专题	大连化学物理研究所_中国科学院大连化学物理研究所
作者单位	1.Chinese Acad Sci, Dalian Inst Chem Phys, Dalian 116023, Peoples R China 2.Dalian Nationalities Univ, Dept Life Sci, Dalian 116600, Peoples R China 3.State Ethn Affairs Commiss, Minist Educ Econ & Technol Dev Zone, Dalian 116600, Peoples R China
推荐引用方式 GB/T 7714	Wang, Lina,Quan, Chunshan,Xiong, Wen,et al. New insight into transmembrane topology of Staphylococcus aureus histidine kinase AgrC[J]. biochimica et biophysica acta-biomembranes,2014,1838(3):988-993.
APA	Wang, Lina,Quan, Chunshan,Xiong, Wen,Qu, Xiaojing,Fan, Shengdi,&Hu, Wenzhong.(2014).New insight into transmembrane topology of Staphylococcus aureus histidine kinase AgrC.biochimica et biophysica acta-biomembranes,1838(3),988-993.
MLA	Wang, Lina,et al."New insight into transmembrane topology of Staphylococcus aureus histidine kinase AgrC".biochimica et biophysica acta-biomembranes 1838.3(2014):988-993.